Regional mapping of myocardial hibernation phenotype in idiopathic end-stage dilated cardiomyopathy

Vincenzo Lionetti, Marco Matteucci, Marco Ribezzo, Dario Di Silvestre, Francesca Brambilla, Silvia Agostini, Pierluigi Mauri, Luigi Padeletti, Alessandro Pingitore, Luisa Delsedime, Mauro Rinaldi, Fabio A. Recchia, Angela Pucci

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Myocardial hibernation (MH) is a well-known feature of human ischaemic cardiomyopathy (ICM), whereas its presence in human idiopathic dilated cardiomyopathy (DCM) is still controversial. We investigated the histological and molecular features of MH in left ventricle (LV) regions of failing DCM or ICM hearts. We examined failing hearts from DCM (n = 11; 41.9 ± 5.45 years; left ventricle-ejection fraction (LV-EF), 18 ± 3.16%) and ICM patients (n = 12; 58.08 ± 1.7 years; LVEF, 21.5 ± 6.08%) undergoing cardiac transplantation, and normal donor hearts (N, n = 8). LV inter-ventricular septum (IVS) and antero-lateral free wall (FW) were transmurally (i.e. sub-epicardial, mesocardial and sub-endocardial layers) analysed. LV glycogen content was shown to be increased in both DCM and ICM as compared with N hearts (P <0.001), with a U-shaped transmural distribution (lower values in mesocardium). Capillary density was homogenously reduced in both DCM and ICM as compared with N (P <0.05 versus N), with a lower decrease independent of the extent of fibrosis in sub-endocardial and sub-epicardial layers of DCM as compared with ICM. HIF1-α and nestin, recognized ischaemic molecular hallmarks, were similarly expressed in DCM-LV and ICM-LV myocardium. The proteomic profile was overlapping by ~50% in DCM and ICM groups. Morphological and molecular features of MH were detected in end-stage ICM as well as in end-stage DCM LV, despite epicardial coronary artery patency and lower fibrosis in DCM hearts. Unravelling the presence of MH in the absence of coronary stenosis may be helpful to design a novel approach in the clinical management of DCM.

Original languageEnglish
Pages (from-to)396-414
Number of pages19
JournalJournal of Cellular and Molecular Medicine
Volume18
Issue number3
DOIs
Publication statusPublished - Mar 2014

Fingerprint

Myocardial Stunning
Dilated Cardiomyopathy
Cardiomyopathies
Phenotype
Heart Ventricles
Fibrosis
Nestin
Ventricular Septum
Coronary Stenosis
Heart Transplantation
Glycogen
Proteomics
Coronary Vessels
Myocardium

Keywords

  • Chronic heart failure
  • Hibernating myocardium
  • Idiopathic dilated cardiomyopathy
  • Ischaemic microenvironment
  • Nestin
  • Pathologic features

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Medicine

Cite this

Lionetti, V., Matteucci, M., Ribezzo, M., Di Silvestre, D., Brambilla, F., Agostini, S., ... Pucci, A. (2014). Regional mapping of myocardial hibernation phenotype in idiopathic end-stage dilated cardiomyopathy. Journal of Cellular and Molecular Medicine, 18(3), 396-414. https://doi.org/10.1111/jcmm.12198

Regional mapping of myocardial hibernation phenotype in idiopathic end-stage dilated cardiomyopathy. / Lionetti, Vincenzo; Matteucci, Marco; Ribezzo, Marco; Di Silvestre, Dario; Brambilla, Francesca; Agostini, Silvia; Mauri, Pierluigi; Padeletti, Luigi; Pingitore, Alessandro; Delsedime, Luisa; Rinaldi, Mauro; Recchia, Fabio A.; Pucci, Angela.

In: Journal of Cellular and Molecular Medicine, Vol. 18, No. 3, 03.2014, p. 396-414.

Research output: Contribution to journalArticle

Lionetti, V, Matteucci, M, Ribezzo, M, Di Silvestre, D, Brambilla, F, Agostini, S, Mauri, P, Padeletti, L, Pingitore, A, Delsedime, L, Rinaldi, M, Recchia, FA & Pucci, A 2014, 'Regional mapping of myocardial hibernation phenotype in idiopathic end-stage dilated cardiomyopathy', Journal of Cellular and Molecular Medicine, vol. 18, no. 3, pp. 396-414. https://doi.org/10.1111/jcmm.12198
Lionetti, Vincenzo ; Matteucci, Marco ; Ribezzo, Marco ; Di Silvestre, Dario ; Brambilla, Francesca ; Agostini, Silvia ; Mauri, Pierluigi ; Padeletti, Luigi ; Pingitore, Alessandro ; Delsedime, Luisa ; Rinaldi, Mauro ; Recchia, Fabio A. ; Pucci, Angela. / Regional mapping of myocardial hibernation phenotype in idiopathic end-stage dilated cardiomyopathy. In: Journal of Cellular and Molecular Medicine. 2014 ; Vol. 18, No. 3. pp. 396-414.
@article{b390137a56c64893991657449f8981c9,
title = "Regional mapping of myocardial hibernation phenotype in idiopathic end-stage dilated cardiomyopathy",
abstract = "Myocardial hibernation (MH) is a well-known feature of human ischaemic cardiomyopathy (ICM), whereas its presence in human idiopathic dilated cardiomyopathy (DCM) is still controversial. We investigated the histological and molecular features of MH in left ventricle (LV) regions of failing DCM or ICM hearts. We examined failing hearts from DCM (n = 11; 41.9 ± 5.45 years; left ventricle-ejection fraction (LV-EF), 18 ± 3.16{\%}) and ICM patients (n = 12; 58.08 ± 1.7 years; LVEF, 21.5 ± 6.08{\%}) undergoing cardiac transplantation, and normal donor hearts (N, n = 8). LV inter-ventricular septum (IVS) and antero-lateral free wall (FW) were transmurally (i.e. sub-epicardial, mesocardial and sub-endocardial layers) analysed. LV glycogen content was shown to be increased in both DCM and ICM as compared with N hearts (P <0.001), with a U-shaped transmural distribution (lower values in mesocardium). Capillary density was homogenously reduced in both DCM and ICM as compared with N (P <0.05 versus N), with a lower decrease independent of the extent of fibrosis in sub-endocardial and sub-epicardial layers of DCM as compared with ICM. HIF1-α and nestin, recognized ischaemic molecular hallmarks, were similarly expressed in DCM-LV and ICM-LV myocardium. The proteomic profile was overlapping by ~50{\%} in DCM and ICM groups. Morphological and molecular features of MH were detected in end-stage ICM as well as in end-stage DCM LV, despite epicardial coronary artery patency and lower fibrosis in DCM hearts. Unravelling the presence of MH in the absence of coronary stenosis may be helpful to design a novel approach in the clinical management of DCM.",
keywords = "Chronic heart failure, Hibernating myocardium, Idiopathic dilated cardiomyopathy, Ischaemic microenvironment, Nestin, Pathologic features",
author = "Vincenzo Lionetti and Marco Matteucci and Marco Ribezzo and {Di Silvestre}, Dario and Francesca Brambilla and Silvia Agostini and Pierluigi Mauri and Luigi Padeletti and Alessandro Pingitore and Luisa Delsedime and Mauro Rinaldi and Recchia, {Fabio A.} and Angela Pucci",
year = "2014",
month = "3",
doi = "10.1111/jcmm.12198",
language = "English",
volume = "18",
pages = "396--414",
journal = "Journal of Cellular and Molecular Medicine",
issn = "1582-1838",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Regional mapping of myocardial hibernation phenotype in idiopathic end-stage dilated cardiomyopathy

AU - Lionetti, Vincenzo

AU - Matteucci, Marco

AU - Ribezzo, Marco

AU - Di Silvestre, Dario

AU - Brambilla, Francesca

AU - Agostini, Silvia

AU - Mauri, Pierluigi

AU - Padeletti, Luigi

AU - Pingitore, Alessandro

AU - Delsedime, Luisa

AU - Rinaldi, Mauro

AU - Recchia, Fabio A.

AU - Pucci, Angela

PY - 2014/3

Y1 - 2014/3

N2 - Myocardial hibernation (MH) is a well-known feature of human ischaemic cardiomyopathy (ICM), whereas its presence in human idiopathic dilated cardiomyopathy (DCM) is still controversial. We investigated the histological and molecular features of MH in left ventricle (LV) regions of failing DCM or ICM hearts. We examined failing hearts from DCM (n = 11; 41.9 ± 5.45 years; left ventricle-ejection fraction (LV-EF), 18 ± 3.16%) and ICM patients (n = 12; 58.08 ± 1.7 years; LVEF, 21.5 ± 6.08%) undergoing cardiac transplantation, and normal donor hearts (N, n = 8). LV inter-ventricular septum (IVS) and antero-lateral free wall (FW) were transmurally (i.e. sub-epicardial, mesocardial and sub-endocardial layers) analysed. LV glycogen content was shown to be increased in both DCM and ICM as compared with N hearts (P <0.001), with a U-shaped transmural distribution (lower values in mesocardium). Capillary density was homogenously reduced in both DCM and ICM as compared with N (P <0.05 versus N), with a lower decrease independent of the extent of fibrosis in sub-endocardial and sub-epicardial layers of DCM as compared with ICM. HIF1-α and nestin, recognized ischaemic molecular hallmarks, were similarly expressed in DCM-LV and ICM-LV myocardium. The proteomic profile was overlapping by ~50% in DCM and ICM groups. Morphological and molecular features of MH were detected in end-stage ICM as well as in end-stage DCM LV, despite epicardial coronary artery patency and lower fibrosis in DCM hearts. Unravelling the presence of MH in the absence of coronary stenosis may be helpful to design a novel approach in the clinical management of DCM.

AB - Myocardial hibernation (MH) is a well-known feature of human ischaemic cardiomyopathy (ICM), whereas its presence in human idiopathic dilated cardiomyopathy (DCM) is still controversial. We investigated the histological and molecular features of MH in left ventricle (LV) regions of failing DCM or ICM hearts. We examined failing hearts from DCM (n = 11; 41.9 ± 5.45 years; left ventricle-ejection fraction (LV-EF), 18 ± 3.16%) and ICM patients (n = 12; 58.08 ± 1.7 years; LVEF, 21.5 ± 6.08%) undergoing cardiac transplantation, and normal donor hearts (N, n = 8). LV inter-ventricular septum (IVS) and antero-lateral free wall (FW) were transmurally (i.e. sub-epicardial, mesocardial and sub-endocardial layers) analysed. LV glycogen content was shown to be increased in both DCM and ICM as compared with N hearts (P <0.001), with a U-shaped transmural distribution (lower values in mesocardium). Capillary density was homogenously reduced in both DCM and ICM as compared with N (P <0.05 versus N), with a lower decrease independent of the extent of fibrosis in sub-endocardial and sub-epicardial layers of DCM as compared with ICM. HIF1-α and nestin, recognized ischaemic molecular hallmarks, were similarly expressed in DCM-LV and ICM-LV myocardium. The proteomic profile was overlapping by ~50% in DCM and ICM groups. Morphological and molecular features of MH were detected in end-stage ICM as well as in end-stage DCM LV, despite epicardial coronary artery patency and lower fibrosis in DCM hearts. Unravelling the presence of MH in the absence of coronary stenosis may be helpful to design a novel approach in the clinical management of DCM.

KW - Chronic heart failure

KW - Hibernating myocardium

KW - Idiopathic dilated cardiomyopathy

KW - Ischaemic microenvironment

KW - Nestin

KW - Pathologic features

UR - http://www.scopus.com/inward/record.url?scp=84894551930&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84894551930&partnerID=8YFLogxK

U2 - 10.1111/jcmm.12198

DO - 10.1111/jcmm.12198

M3 - Article

VL - 18

SP - 396

EP - 414

JO - Journal of Cellular and Molecular Medicine

JF - Journal of Cellular and Molecular Medicine

SN - 1582-1838

IS - 3

ER -