Regional variations in binding capacities at μ-, δ- and κ-opioid sites in membrane suspensions from rabbit brain

Paola Petrillo, Antonio La Regina, Massimo Sbacchi, Alessandra Tavani, Linda E. Robson, Hans W. Kosterlitz

Research output: Contribution to journalArticlepeer-review

Abstract

The highest maximum binding capacities at the μ-sites of rabbit brain are in the striatum, with intermediate levels in the diencephalon, mesencephalon, cerebellum and cortex and low level in the pons-medulla and hippocampus. For the δ-site the highest maximum binding capacity is also in the striatum; there are almost equally low levels in the other brain regions. At the κ-sites the maximum binding capacities are highest in the diencephalon; there are intermediate levels in the cortex and striatum, and low levels in the mesencephalon, cerebellum, hippocampus and pons-medulla. The KD values lack reproducibility; there are no regional variations at the κ-site as estimated with [3H](-)-bremazocine, but the possibility cannot be excluded that there are regional variations in the KD values for [3H][D-Ala2, MePhe4, Gly-ol5]enkephalin at the μ-site or for [3H][D-Ala2,D-Leu5]enkephalin at the δ-site. It may be important to use saturation analysis in future investigations of the distributions of the binding sites.

Original languageEnglish
Pages (from-to)213-217
Number of pages5
JournalEuropean Journal of Pharmacology
Volume166
Issue number2
DOIs
Publication statusPublished - Jul 18 1989

Keywords

  • (Binding capacities
  • Binding affinities
  • Brain regions
  • Rabbit
  • δ-
  • κ-Opioid binding sites
  • μ-

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

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