Abstract
MUC1 is a large surface glycoprotein expressed by epithelial cells, which is overexpressed and aberrantly glycosylated in carcinomas. MUC1 is involved in epithelial cell interactions and appears to function as a signal-transducing molecule. The finding that MUC1 can also be expressed in the haematopoietic lineages prompted us to further investigate the possible function(s) of this molecule in haematopoietic cells. In bone marrow differentiating cells, MUC1 was strongly and selectively expressed during erythropoiesis; it was also weakly expressed during megakaryocytopoiesis and granulomonocytopoiesis; however, no correlation between MUC1 and differentiation marker expression was observed in these lineages. In vitro CD34+ cells, induced towards erythroid differentiation, acquired MUC1 transiently, while expressing increasing levels of the lineage marker glycophorin A. MUC1 was absent in the circulating erythrocytes. During erythropoiesis, MUC1 expression was transcriptionally regulated and the molecule underwent phosphorylation. To investigate the possible role of MUC1 during erythropoiesis, we studied the ability of MUC1 to act as ligand for cell-cell interaction. The sialylated MUC1 glycoforms selectively expressed on erythroid cells were able to bind the macrophage-restricted molecule sialoadhesin. These results suggest that MUC1 can function as a cross-talk molecule between the erythroblasts and the surrounding cells during erythropoiesis.
| Original language | English |
|---|---|
| Pages (from-to) | 344-352 |
| Number of pages | 9 |
| Journal | British Journal of Haematology |
| Volume | 120 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 2003 |
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Keywords
- Cell-cell interaction
- Erythropoiesis
- Haematopoiesis
- MUC1
- Sialoadhesin
ASJC Scopus subject areas
- Hematology
Cite this
Regulated expression of MUC1 epithelial antigen in erythropoiesis. / Rughetti, Aurelia; Biffoni, Mauro; Pierelli, Luca; Rahimi, Hassan; Bonanno, Giuseppina; Barachini, Serena; Pellicciotta, Ilenia; Napoletano, Chiara; Pescarmona, Edoardo; Del Nero, Angelo; Pignoloni, Patrizia; Frati, Luigi; Nuti, Marianna.
In: British Journal of Haematology, Vol. 120, No. 2, 01.2003, p. 344-352.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regulated expression of MUC1 epithelial antigen in erythropoiesis
AU - Rughetti, Aurelia
AU - Biffoni, Mauro
AU - Pierelli, Luca
AU - Rahimi, Hassan
AU - Bonanno, Giuseppina
AU - Barachini, Serena
AU - Pellicciotta, Ilenia
AU - Napoletano, Chiara
AU - Pescarmona, Edoardo
AU - Del Nero, Angelo
AU - Pignoloni, Patrizia
AU - Frati, Luigi
AU - Nuti, Marianna
PY - 2003/1
Y1 - 2003/1
N2 - MUC1 is a large surface glycoprotein expressed by epithelial cells, which is overexpressed and aberrantly glycosylated in carcinomas. MUC1 is involved in epithelial cell interactions and appears to function as a signal-transducing molecule. The finding that MUC1 can also be expressed in the haematopoietic lineages prompted us to further investigate the possible function(s) of this molecule in haematopoietic cells. In bone marrow differentiating cells, MUC1 was strongly and selectively expressed during erythropoiesis; it was also weakly expressed during megakaryocytopoiesis and granulomonocytopoiesis; however, no correlation between MUC1 and differentiation marker expression was observed in these lineages. In vitro CD34+ cells, induced towards erythroid differentiation, acquired MUC1 transiently, while expressing increasing levels of the lineage marker glycophorin A. MUC1 was absent in the circulating erythrocytes. During erythropoiesis, MUC1 expression was transcriptionally regulated and the molecule underwent phosphorylation. To investigate the possible role of MUC1 during erythropoiesis, we studied the ability of MUC1 to act as ligand for cell-cell interaction. The sialylated MUC1 glycoforms selectively expressed on erythroid cells were able to bind the macrophage-restricted molecule sialoadhesin. These results suggest that MUC1 can function as a cross-talk molecule between the erythroblasts and the surrounding cells during erythropoiesis.
AB - MUC1 is a large surface glycoprotein expressed by epithelial cells, which is overexpressed and aberrantly glycosylated in carcinomas. MUC1 is involved in epithelial cell interactions and appears to function as a signal-transducing molecule. The finding that MUC1 can also be expressed in the haematopoietic lineages prompted us to further investigate the possible function(s) of this molecule in haematopoietic cells. In bone marrow differentiating cells, MUC1 was strongly and selectively expressed during erythropoiesis; it was also weakly expressed during megakaryocytopoiesis and granulomonocytopoiesis; however, no correlation between MUC1 and differentiation marker expression was observed in these lineages. In vitro CD34+ cells, induced towards erythroid differentiation, acquired MUC1 transiently, while expressing increasing levels of the lineage marker glycophorin A. MUC1 was absent in the circulating erythrocytes. During erythropoiesis, MUC1 expression was transcriptionally regulated and the molecule underwent phosphorylation. To investigate the possible role of MUC1 during erythropoiesis, we studied the ability of MUC1 to act as ligand for cell-cell interaction. The sialylated MUC1 glycoforms selectively expressed on erythroid cells were able to bind the macrophage-restricted molecule sialoadhesin. These results suggest that MUC1 can function as a cross-talk molecule between the erythroblasts and the surrounding cells during erythropoiesis.
KW - Cell-cell interaction
KW - Erythropoiesis
KW - Haematopoiesis
KW - MUC1
KW - Sialoadhesin
UR - http://www.scopus.com/inward/record.url?scp=0037278398&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037278398&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2141.2003.04038.x
DO - 10.1046/j.1365-2141.2003.04038.x
M3 - Article
C2 - 12542497
AN - SCOPUS:0037278398
VL - 120
SP - 344
EP - 352
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 2
ER -