Regulated expression of vascular cell adhesion molecule-1 in human malignant melanoma

Nives Jonjic, Inés Martìn-Padura, Teresa Pollicino, Sergio Bernasconi, Petr Jílek, Aldo Bigotti, Roberta Mortarini, Andrea Anichini, Giorgio Parmiani, Francesco Colotta, Elisabetta Dejana, Alberto Mantovani, Pier Giorgio Natali

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Abstract

Expression of the endothelial adhesion molecule VCAM-1 was studied in human malignant melanoma lines by flout cytometry. Clones 214 and 2/14 (derived from the same lesion) had appreciable levels of VCAM-1 expression, whereas clone 2/21 and the lines A2058, Mel24, and A375 were negative. Clone 2114 was selected for further analysis. Exposure to tumor necrosis factor (TNF) markedly augmented VCAM-1 on melanoma cells. Surface VCAM-1 was associated with expression of specific transcripts that were augmented by TNF. Analysis by reverse transcriptase and polymerase chain reaction using appropriate primers revealed that TNF-stimulated melanoma cells expressed both 7 and 6 immunoglobulin domain transcripts with predominance of the longer species. Tumor necrosis factor-stimulated melanoma cells bound more VLA·4-expressing cells (melanoma and monocytes) than resting tumor cells and anti-VCAM-1 monoclonal antibodies significantly inhibited binding, thus suggesting that surface VCAM-1 on melanoma is functional. Analysis of melanoma tissue sections demonstrated that VCAM-1 is not a marker of transformation of melanocytes because it can be detected in benign nevi. Although, unlike ICAM-1, VCAM-1 is not correlated with tumor progression, its expression in a fraction of primary Melanomas indicates that it may play a role in regulating host immune response and homotypic interactions in some malignant melanomas.

Original languageEnglish
Pages (from-to)1323-1330
Number of pages8
JournalAmerican Journal of Pathology
Volume141
Issue number6
Publication statusPublished - Dec 1992

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Vascular Cell Adhesion Molecule-1
Melanoma
Tumor Necrosis Factor-alpha
Clone Cells
Nevus
Melanocytes
Intercellular Adhesion Molecule-1
Reverse Transcriptase Polymerase Chain Reaction
Monocytes
Neoplasms
Monoclonal Antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Jonjic, N., Martìn-Padura, I., Pollicino, T., Bernasconi, S., Jílek, P., Bigotti, A., ... Natali, P. G. (1992). Regulated expression of vascular cell adhesion molecule-1 in human malignant melanoma. American Journal of Pathology, 141(6), 1323-1330.

Regulated expression of vascular cell adhesion molecule-1 in human malignant melanoma. / Jonjic, Nives; Martìn-Padura, Inés; Pollicino, Teresa; Bernasconi, Sergio; Jílek, Petr; Bigotti, Aldo; Mortarini, Roberta; Anichini, Andrea; Parmiani, Giorgio; Colotta, Francesco; Dejana, Elisabetta; Mantovani, Alberto; Natali, Pier Giorgio.

In: American Journal of Pathology, Vol. 141, No. 6, 12.1992, p. 1323-1330.

Research output: Contribution to journalArticle

Jonjic, N, Martìn-Padura, I, Pollicino, T, Bernasconi, S, Jílek, P, Bigotti, A, Mortarini, R, Anichini, A, Parmiani, G, Colotta, F, Dejana, E, Mantovani, A & Natali, PG 1992, 'Regulated expression of vascular cell adhesion molecule-1 in human malignant melanoma', American Journal of Pathology, vol. 141, no. 6, pp. 1323-1330.
Jonjic N, Martìn-Padura I, Pollicino T, Bernasconi S, Jílek P, Bigotti A et al. Regulated expression of vascular cell adhesion molecule-1 in human malignant melanoma. American Journal of Pathology. 1992 Dec;141(6):1323-1330.
Jonjic, Nives ; Martìn-Padura, Inés ; Pollicino, Teresa ; Bernasconi, Sergio ; Jílek, Petr ; Bigotti, Aldo ; Mortarini, Roberta ; Anichini, Andrea ; Parmiani, Giorgio ; Colotta, Francesco ; Dejana, Elisabetta ; Mantovani, Alberto ; Natali, Pier Giorgio. / Regulated expression of vascular cell adhesion molecule-1 in human malignant melanoma. In: American Journal of Pathology. 1992 ; Vol. 141, No. 6. pp. 1323-1330.
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