Regulated expression of vascular cell adhesion molecule-1 in human malignant melanoma

Nives Jonjic, Inés Martìn-Padura, Teresa Pollicino, Sergio Bernasconi, Petr Jílek, Aldo Bigotti, Roberta Mortarini, Andrea Anichini, Giorgio Parmiani, Francesco Colotta, Elisabetta Dejana, Alberto Mantovani, Pier Giorgio Natali

Research output: Contribution to journalArticle

Abstract

Expression of the endothelial adhesion molecule VCAM-1 was studied in human malignant melanoma lines by flout cytometry. Clones 214 and 2/14 (derived from the same lesion) had appreciable levels of VCAM-1 expression, whereas clone 2/21 and the lines A2058, Mel24, and A375 were negative. Clone 2114 was selected for further analysis. Exposure to tumor necrosis factor (TNF) markedly augmented VCAM-1 on melanoma cells. Surface VCAM-1 was associated with expression of specific transcripts that were augmented by TNF. Analysis by reverse transcriptase and polymerase chain reaction using appropriate primers revealed that TNF-stimulated melanoma cells expressed both 7 and 6 immunoglobulin domain transcripts with predominance of the longer species. Tumor necrosis factor-stimulated melanoma cells bound more VLA·4-expressing cells (melanoma and monocytes) than resting tumor cells and anti-VCAM-1 monoclonal antibodies significantly inhibited binding, thus suggesting that surface VCAM-1 on melanoma is functional. Analysis of melanoma tissue sections demonstrated that VCAM-1 is not a marker of transformation of melanocytes because it can be detected in benign nevi. Although, unlike ICAM-1, VCAM-1 is not correlated with tumor progression, its expression in a fraction of primary Melanomas indicates that it may play a role in regulating host immune response and homotypic interactions in some malignant melanomas.

Original languageEnglish
Pages (from-to)1323-1330
Number of pages8
JournalAmerican Journal of Pathology
Volume141
Issue number6
Publication statusPublished - Dec 1992

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Jonjic, N., Martìn-Padura, I., Pollicino, T., Bernasconi, S., Jílek, P., Bigotti, A., Mortarini, R., Anichini, A., Parmiani, G., Colotta, F., Dejana, E., Mantovani, A., & Natali, P. G. (1992). Regulated expression of vascular cell adhesion molecule-1 in human malignant melanoma. American Journal of Pathology, 141(6), 1323-1330.