Regulation and patterns of endogenous and exogenous gene expression during differentiation of embryonal carcinoma cells

S. Astigiano, M. I. Sherman, P. Abarzua

Research output: Contribution to journalArticlepeer-review

Abstract

Embryonal carcinoma (EC) cells offer an interesting model system for evaluating differentiation because the cells are pluripotent, thus resembling germ cells and embryonic stem cells, and because a number of agents have been defined that are capable of promoting the differentiation of these cells. This chapter examines how EC cells might be triggered to differentiate, with emphasis on retinoic acid because this compound is a potent, naturally occurring inducer that has been studied extensively in this system. The nature of alterations in gene expression during EC cell differentiation is reviewed from the perspective of evaluating whether these changes are likely to be responsible for, or a result of, the differentiation event. Finally, we consider in molecular terms why EC cells, but not their differentiated derivatives, are refractory to the expression of many viral genomes following infection. Based upon these studies, we propose that fundamental changes in gene expression that are observed when differentiation is triggered in EC cells are likely to be due to the disappearance or neutralization of strong repressor elements.

Original languageEnglish
Pages (from-to)25-38
Number of pages14
JournalEnvironmental Health Perspectives
Volume80
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health

Fingerprint Dive into the research topics of 'Regulation and patterns of endogenous and exogenous gene expression during differentiation of embryonal carcinoma cells'. Together they form a unique fingerprint.

Cite this