Regulation of adhE (encoding ethanol oxidoreductase) by the fis protein in Escherichia coli

Jorge Membrillo-Hernández, Ohsuk Kwon, Peter De Wulf, Steven E. Finkel, E. C C Lin

Research output: Contribution to journalArticle

Abstract

The adhE gene of Escherichia coil encodes a multifunctional ethanol oxidoreductase whose expression is 10-fold higher under anaerobic than aerobic conditions. Transcription of the gene is under the negative control of the Cra (catabolite repressor- activator) protein, whereas translation of the adhE mRNA requires processing by RNase III. In this report, we show that the expression of adhE also depends on the Fis (factor for inversion stimulation) protein. A strain bearing a fis::kan null allele failed to grow anaerobically on glucose solely because of inadequate adhE transcription. However, fis expression itself is not under redox control. Sequence inspection of the adhE promoter revealed three potential Fis binding sites. Electrophoretic mobility shift analysis, using purified Fis protein and adhE promoter DNA, showed three different complexes.

Original languageEnglish
Pages (from-to)7390-7393
Number of pages4
JournalJournal of Bacteriology
Volume181
Issue number23
Publication statusPublished - Dec 1999

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Fingerprint Dive into the research topics of 'Regulation of adhE (encoding ethanol oxidoreductase) by the fis protein in Escherichia coli'. Together they form a unique fingerprint.

Cite this