Regulation of BRCA1 transcription by specific single-stranded DNA binding factors

Sanjay Thakur, Tatsuya Nakamura, George Calin, Andrea Russo, Joseph F. Tamburrino, Masayoshi Shimizu, Gustavo Baldassarre, Sabrina Battista, Alfredo Fusco, Richard P. Wassell, Garret Dubois, Hansjuerg Alder, Carlo M. Croce

Research output: Contribution to journalArticle

Abstract

Since the majority of high-grade breast cancers express reduced levels of BRCA1 mRNA, we investigated the factors regulating BRCA1 transcription. Factors with specific affinity for the previously identified positive regulatory region (PRR) in the BRCA1 promoter were purified from whole-cell extracts. Identified proteins included replication protein A and a series of related factors with affinity for the sense strand of PRR. A subset of the identified factors activated the BRCA1 promoter. Identification of these families of proteins regulating the BRCA1 promoter represents an important step in the comprehension of the mechanisms responsible for breast cancer development.

Original languageEnglish
Pages (from-to)3774-3787
Number of pages14
JournalMolecular and Cellular Biology
Volume23
Issue number11
DOIs
Publication statusPublished - Jun 2003

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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    Thakur, S., Nakamura, T., Calin, G., Russo, A., Tamburrino, J. F., Shimizu, M., Baldassarre, G., Battista, S., Fusco, A., Wassell, R. P., Dubois, G., Alder, H., & Croce, C. M. (2003). Regulation of BRCA1 transcription by specific single-stranded DNA binding factors. Molecular and Cellular Biology, 23(11), 3774-3787. https://doi.org/10.1128/MCB.23.11.3774-3787.2003