Regulation of histone acetyltransferases p300 and PCAF by the bHLH protein twist and adenoviral oncoprotein E1A

Yasuo Hamamori, Vittorio Sartorelli, Vasily Ogryzko, Pier Lorenzo Puri, Hung Yi Wu, Jean Y J Wang, Yoshihiro Nakatani, Larry Kedes

Research output: Contribution to journalArticle

Abstract

Histone acetyltransferases (HAT) play a critical role in transcriptional control by relieving repressive effects of chromatin, and yet how HATS themselves are regulated remains largely unknown. Here, it is shown that Twist directly binds two independent HAT domains of acetyltransferases, p300 and p300/CBP-associated factor (PCAF), and directly regulates their HAT activities. The N terminus of Twist is a primary domain interacting with both acetyltransferases, and the same domain is required for inhibition of p300- dependent transcription by Twist. Adenovirus E1A protein mimics the effects of Twist by inhibiting the HAT activities of p300 and PCAF. These findings establish a cogent argument for considering the HAT domains as a direct target for acetyltransferase regulation by both a cellular transcription factor and a viral oncoprotein.

Original languageEnglish
Pages (from-to)405-413
Number of pages9
JournalCell
Volume96
Issue number3
DOIs
Publication statusPublished - Feb 5 1999

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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    Hamamori, Y., Sartorelli, V., Ogryzko, V., Puri, P. L., Wu, H. Y., Wang, J. Y. J., Nakatani, Y., & Kedes, L. (1999). Regulation of histone acetyltransferases p300 and PCAF by the bHLH protein twist and adenoviral oncoprotein E1A. Cell, 96(3), 405-413. https://doi.org/10.1016/S0092-8674(00)80553-X