Regulation of human c-Abl tyrosine kinase activity in Xenopus oocytes and acceleration of progesterone-induce G2/M transition by oncogenic forms

Karel Dorey, Daniela Barilá, Anne Claude Gavin, Angel R. Nebreda, Giulio Superti-Furga

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Deregulated activity of the Abl protein tyrosine kinase is oncogenic in humans and in animals. The normal cellular form of the enzyme is maintained at a low state of activity by mechanisms that have not yet been entirely elucidated. In particular, little is known about the trans-acting cellular factors involved. We have tested the activity of human c-Abl microinjected into oocytes of Xenopus laevis. In contrast to versions of Abl capable of transforming mammalian cells, which were highly active when introduced into oocytes, the activity of wild type c-Abl was inhibited. Oncogenic forms of Abl efficiently enhanced the ability of Xenopos oocytes to enter M phase following stimulation by progesterone. Abl-enhanced maturation was normal as judged by accumulation of Mos as well as activation of MAP kinase and Cdc2/CyclinB (MPF). Concomitant with maturation and activation of these kinases, Abl became extensively phosphorylated. Altogether, this suggests that an SH3 domain-dependent Abl regulation mechanism similar to the one observed in mammalian cells operates in Xenopus oocytes. Maturation enhancement by microinjection into Xenopus oocytes represents a useful novel assay for analyzing Abl activity. Moreover, the Xenopus oocyte may be a convenient source of trans-acting Abl regulators for biochemical studies.

Original languageEnglish
Pages (from-to)223-230
Number of pages8
JournalBiological Chemistry
Volume380
Issue number2
Publication statusPublished - Feb 1999

Fingerprint

Xenopus
Protein-Tyrosine Kinases
Oocytes
Progesterone
Phosphotransferases
Chemical activation
Cells
Assays
Animals
Enzymes
Trans-Activators
src Homology Domains
Xenopus laevis
Microinjections
Human Activities
Cell Division

Keywords

  • Cdc2
  • Cell-cycle
  • MAP kinase
  • Oncogene
  • SH3 domain
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry

Cite this

Regulation of human c-Abl tyrosine kinase activity in Xenopus oocytes and acceleration of progesterone-induce G2/M transition by oncogenic forms. / Dorey, Karel; Barilá, Daniela; Gavin, Anne Claude; Nebreda, Angel R.; Superti-Furga, Giulio.

In: Biological Chemistry, Vol. 380, No. 2, 02.1999, p. 223-230.

Research output: Contribution to journalArticle

Dorey, Karel ; Barilá, Daniela ; Gavin, Anne Claude ; Nebreda, Angel R. ; Superti-Furga, Giulio. / Regulation of human c-Abl tyrosine kinase activity in Xenopus oocytes and acceleration of progesterone-induce G2/M transition by oncogenic forms. In: Biological Chemistry. 1999 ; Vol. 380, No. 2. pp. 223-230.
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