Abstract
According to the pattern of IgM biosynthesis (membrane expression and secretion), human B cell chronic lymphocytic leukemias (B-CLLs) were subgrouped into four classes, namely: class I: membrane- secretion-; class II: membrane+ secretion-; class III: membrane+ secretion+; class IV: membrane- secretion+. Abundant membrane μ chain mRNA was present in cells from all cases, indicating that translational and/or post translational events were responsible for the absence of surface IgM in classes I and IV. Similarly, post translational events blocked IgM secretion in non secreting B-CLL cells. In B-CLLs from classes I, II and III, TPA induced IgM secretion by up-regulating secretory μ chain mRNA. By contrast, in normal B cells, TPA induced down-regulation of the secretory form of Ig mRNA, irrespective of the maturational stage of the cell. These observations indicate that IgM biosynthesis is modulated differently by TPA in normal and malignant B cells.
| Original language | English |
|---|---|
| Pages (from-to) | 1105-1111 |
| Number of pages | 7 |
| Journal | Leukemia Research |
| Volume | 13 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 1989 |
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Keywords
- Chronic lymphocytic leukemia
- IgM
- membrane expression
- secretion
- TPA
ASJC Scopus subject areas
- Cancer Research
- Hematology
- Oncology
Cite this
Regulation of IgM biosynthesis in human chronic lymphocytic leukemia. Normal and neoplastic B cells respond differently to TPA. / Guazzi, Stefania; Sitia, Roberto; Rubartelli, Anna.
In: Leukemia Research, Vol. 13, No. 12, 1989, p. 1105-1111.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regulation of IgM biosynthesis in human chronic lymphocytic leukemia. Normal and neoplastic B cells respond differently to TPA
AU - Guazzi, Stefania
AU - Sitia, Roberto
AU - Rubartelli, Anna
PY - 1989
Y1 - 1989
N2 - According to the pattern of IgM biosynthesis (membrane expression and secretion), human B cell chronic lymphocytic leukemias (B-CLLs) were subgrouped into four classes, namely: class I: membrane- secretion-; class II: membrane+ secretion-; class III: membrane+ secretion+; class IV: membrane- secretion+. Abundant membrane μ chain mRNA was present in cells from all cases, indicating that translational and/or post translational events were responsible for the absence of surface IgM in classes I and IV. Similarly, post translational events blocked IgM secretion in non secreting B-CLL cells. In B-CLLs from classes I, II and III, TPA induced IgM secretion by up-regulating secretory μ chain mRNA. By contrast, in normal B cells, TPA induced down-regulation of the secretory form of Ig mRNA, irrespective of the maturational stage of the cell. These observations indicate that IgM biosynthesis is modulated differently by TPA in normal and malignant B cells.
AB - According to the pattern of IgM biosynthesis (membrane expression and secretion), human B cell chronic lymphocytic leukemias (B-CLLs) were subgrouped into four classes, namely: class I: membrane- secretion-; class II: membrane+ secretion-; class III: membrane+ secretion+; class IV: membrane- secretion+. Abundant membrane μ chain mRNA was present in cells from all cases, indicating that translational and/or post translational events were responsible for the absence of surface IgM in classes I and IV. Similarly, post translational events blocked IgM secretion in non secreting B-CLL cells. In B-CLLs from classes I, II and III, TPA induced IgM secretion by up-regulating secretory μ chain mRNA. By contrast, in normal B cells, TPA induced down-regulation of the secretory form of Ig mRNA, irrespective of the maturational stage of the cell. These observations indicate that IgM biosynthesis is modulated differently by TPA in normal and malignant B cells.
KW - Chronic lymphocytic leukemia
KW - IgM
KW - membrane expression
KW - secretion
KW - TPA
UR - http://www.scopus.com/inward/record.url?scp=0024787444&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024787444&partnerID=8YFLogxK
U2 - 10.1016/0145-2126(89)90156-2
DO - 10.1016/0145-2126(89)90156-2
M3 - Article
C2 - 2615467
AN - SCOPUS:0024787444
VL - 13
SP - 1105
EP - 1111
JO - Leukemia Research
JF - Leukemia Research
SN - 0145-2126
IS - 12
ER -