Regulation of iodide uptake and sodium/iodide symporter expression in the MCF-7 human breast cancer cell line

Franco Arturi, Elisabetta Ferretti, Ivan Presta, Tiziana Mattei, Angela Scipioni, Daniela Scarpelli, Rocco Bruno, Ludovic Lacroix, Emanuele Tosi, Alberto Gulino, Diego Russo, Sebastiano Filetti

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Sodium/iodide symporter (NIS) expression has recently been described in human breast cancer, with emphasis on its potential exploitation for the treatment of these tumors with radioiodine. In this study, we analyzed the regulation of NIS expression and function in the MCF-7 human breast cancer cell line. Cell exposure to insulin, IGF-I, IGF-II, or prolactin induced significant increases in 125I uptake and the expression of both NIS mRNA and NIS protein. The latter increases were evident after 6 and 12 h of hormonal stimulation, respectively. In immunocytochemistry studies, NIS was detected mainly in the plasma membrane of MCF-7 cells. A low but significant increase in iodide uptake was produced by treatment with activators of the adenylyl cyclase (cAMP) or protein kinase C pathways. Our study demonstrates that: 1) MCF-7 breast cancer cells are capable of active iodide transport that can be stimulated by insulin, IGF-I, IGF-II, or prolactin; 2) both NIS transcript and protein are expressed in these cells, and this expression is also hormonally stimulated; and 3) MCF-7 iodide transport and NIS expression may be influenced by the activation of cAMP or protein kinase C-dependent signaling. These findings increase our understanding of the molecular mechanisms that regulate NIS expression in breast cancer cells, information that is fundamental for future research aimed at the development of targeted radioiodide treatment for this type of cancer.

Original languageEnglish
Pages (from-to)2321-2326
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume90
Issue number4
DOIs
Publication statusPublished - Apr 2005

Fingerprint

Iodides
Insulin-Like Growth Factor II
Cells
Breast Neoplasms
Insulin-Like Growth Factor I
Cell Line
Prolactin
Protein Kinase C
Insulin
Cell membranes
Adenylyl Cyclases
Tumors
Active Biological Transport
MCF-7 Cells
Chemical activation
Neoplasms
Messenger RNA
Therapeutics
Immunohistochemistry
Cell Membrane

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Regulation of iodide uptake and sodium/iodide symporter expression in the MCF-7 human breast cancer cell line. / Arturi, Franco; Ferretti, Elisabetta; Presta, Ivan; Mattei, Tiziana; Scipioni, Angela; Scarpelli, Daniela; Bruno, Rocco; Lacroix, Ludovic; Tosi, Emanuele; Gulino, Alberto; Russo, Diego; Filetti, Sebastiano.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 90, No. 4, 04.2005, p. 2321-2326.

Research output: Contribution to journalArticle

Arturi, F, Ferretti, E, Presta, I, Mattei, T, Scipioni, A, Scarpelli, D, Bruno, R, Lacroix, L, Tosi, E, Gulino, A, Russo, D & Filetti, S 2005, 'Regulation of iodide uptake and sodium/iodide symporter expression in the MCF-7 human breast cancer cell line', Journal of Clinical Endocrinology and Metabolism, vol. 90, no. 4, pp. 2321-2326. https://doi.org/10.1210/jc.2004-1562
Arturi, Franco ; Ferretti, Elisabetta ; Presta, Ivan ; Mattei, Tiziana ; Scipioni, Angela ; Scarpelli, Daniela ; Bruno, Rocco ; Lacroix, Ludovic ; Tosi, Emanuele ; Gulino, Alberto ; Russo, Diego ; Filetti, Sebastiano. / Regulation of iodide uptake and sodium/iodide symporter expression in the MCF-7 human breast cancer cell line. In: Journal of Clinical Endocrinology and Metabolism. 2005 ; Vol. 90, No. 4. pp. 2321-2326.
@article{a7063ed663fc440f918dbe9db1431916,
title = "Regulation of iodide uptake and sodium/iodide symporter expression in the MCF-7 human breast cancer cell line",
abstract = "Sodium/iodide symporter (NIS) expression has recently been described in human breast cancer, with emphasis on its potential exploitation for the treatment of these tumors with radioiodine. In this study, we analyzed the regulation of NIS expression and function in the MCF-7 human breast cancer cell line. Cell exposure to insulin, IGF-I, IGF-II, or prolactin induced significant increases in 125I uptake and the expression of both NIS mRNA and NIS protein. The latter increases were evident after 6 and 12 h of hormonal stimulation, respectively. In immunocytochemistry studies, NIS was detected mainly in the plasma membrane of MCF-7 cells. A low but significant increase in iodide uptake was produced by treatment with activators of the adenylyl cyclase (cAMP) or protein kinase C pathways. Our study demonstrates that: 1) MCF-7 breast cancer cells are capable of active iodide transport that can be stimulated by insulin, IGF-I, IGF-II, or prolactin; 2) both NIS transcript and protein are expressed in these cells, and this expression is also hormonally stimulated; and 3) MCF-7 iodide transport and NIS expression may be influenced by the activation of cAMP or protein kinase C-dependent signaling. These findings increase our understanding of the molecular mechanisms that regulate NIS expression in breast cancer cells, information that is fundamental for future research aimed at the development of targeted radioiodide treatment for this type of cancer.",
author = "Franco Arturi and Elisabetta Ferretti and Ivan Presta and Tiziana Mattei and Angela Scipioni and Daniela Scarpelli and Rocco Bruno and Ludovic Lacroix and Emanuele Tosi and Alberto Gulino and Diego Russo and Sebastiano Filetti",
year = "2005",
month = "4",
doi = "10.1210/jc.2004-1562",
language = "English",
volume = "90",
pages = "2321--2326",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "The Endocrine Society",
number = "4",

}

TY - JOUR

T1 - Regulation of iodide uptake and sodium/iodide symporter expression in the MCF-7 human breast cancer cell line

AU - Arturi, Franco

AU - Ferretti, Elisabetta

AU - Presta, Ivan

AU - Mattei, Tiziana

AU - Scipioni, Angela

AU - Scarpelli, Daniela

AU - Bruno, Rocco

AU - Lacroix, Ludovic

AU - Tosi, Emanuele

AU - Gulino, Alberto

AU - Russo, Diego

AU - Filetti, Sebastiano

PY - 2005/4

Y1 - 2005/4

N2 - Sodium/iodide symporter (NIS) expression has recently been described in human breast cancer, with emphasis on its potential exploitation for the treatment of these tumors with radioiodine. In this study, we analyzed the regulation of NIS expression and function in the MCF-7 human breast cancer cell line. Cell exposure to insulin, IGF-I, IGF-II, or prolactin induced significant increases in 125I uptake and the expression of both NIS mRNA and NIS protein. The latter increases were evident after 6 and 12 h of hormonal stimulation, respectively. In immunocytochemistry studies, NIS was detected mainly in the plasma membrane of MCF-7 cells. A low but significant increase in iodide uptake was produced by treatment with activators of the adenylyl cyclase (cAMP) or protein kinase C pathways. Our study demonstrates that: 1) MCF-7 breast cancer cells are capable of active iodide transport that can be stimulated by insulin, IGF-I, IGF-II, or prolactin; 2) both NIS transcript and protein are expressed in these cells, and this expression is also hormonally stimulated; and 3) MCF-7 iodide transport and NIS expression may be influenced by the activation of cAMP or protein kinase C-dependent signaling. These findings increase our understanding of the molecular mechanisms that regulate NIS expression in breast cancer cells, information that is fundamental for future research aimed at the development of targeted radioiodide treatment for this type of cancer.

AB - Sodium/iodide symporter (NIS) expression has recently been described in human breast cancer, with emphasis on its potential exploitation for the treatment of these tumors with radioiodine. In this study, we analyzed the regulation of NIS expression and function in the MCF-7 human breast cancer cell line. Cell exposure to insulin, IGF-I, IGF-II, or prolactin induced significant increases in 125I uptake and the expression of both NIS mRNA and NIS protein. The latter increases were evident after 6 and 12 h of hormonal stimulation, respectively. In immunocytochemistry studies, NIS was detected mainly in the plasma membrane of MCF-7 cells. A low but significant increase in iodide uptake was produced by treatment with activators of the adenylyl cyclase (cAMP) or protein kinase C pathways. Our study demonstrates that: 1) MCF-7 breast cancer cells are capable of active iodide transport that can be stimulated by insulin, IGF-I, IGF-II, or prolactin; 2) both NIS transcript and protein are expressed in these cells, and this expression is also hormonally stimulated; and 3) MCF-7 iodide transport and NIS expression may be influenced by the activation of cAMP or protein kinase C-dependent signaling. These findings increase our understanding of the molecular mechanisms that regulate NIS expression in breast cancer cells, information that is fundamental for future research aimed at the development of targeted radioiodide treatment for this type of cancer.

UR - http://www.scopus.com/inward/record.url?scp=20244387416&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20244387416&partnerID=8YFLogxK

U2 - 10.1210/jc.2004-1562

DO - 10.1210/jc.2004-1562

M3 - Article

C2 - 15623812

AN - SCOPUS:20244387416

VL - 90

SP - 2321

EP - 2326

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 4

ER -