Abstract
In the present study, by comparing the responses in wild-type mice (iNOSWT) and mice lacking (iNOSKO) the inducible (or type 2) nitric oxide synthase (iNOS), we investigated the correlation between endogenous nitric oxide (NO) and prostaglandin (PG) generation in carrageenan-induced pleurisy. The inflammatory response in iNOSKO mice was significantly reduced in respect to iNOSWT animals, as demonstrated by the exudate volume (-63%) and numbers of infiltrating cells (-62%). The levels of NOx in the pleural exudate from carrageenan-treated mice were significantly (p <0.01) decreased in iNOSKO mice (16 ± 7.6 nmoles/mice) compared to iNOSWT animals (133 ± 9 nmoles/mice). Similarly, the amounts of PGE2 in the pleural exudates of carrageenan-treated animals were significantly (p <0.01) lower in iNOSKO compared to iNOSWT mice (120 ± 20 pg/mice vs. 308 ± 51 pg/mice). Also the amounts of 6-keto-PGF1α produced by lungs from carrageenan-treated iNOSKO mice (1.01 ± 0.10 ng/tissue mg) were significantly (p <0.01) reduced compared to iNOSWT carrageenan-treated mice (2.1 ± 0.09 ng/tissue mg). In conclusion our results confirm, by the use of iNOSKO mice that in carrageenan-induced pleurisy NO positively modulates PG biosynthesis.
| Original language | English |
|---|---|
| Pages (from-to) | 1199-1208 |
| Number of pages | 10 |
| Journal | Life Sciences |
| Volume | 72 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Jan 24 2003 |
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Keywords
- Carrageenan
- Cyclooxygenase
- Inflammation
- Nitric oxide
- Prostaglandins
ASJC Scopus subject areas
- Pharmacology
Cite this
Regulation of prostaglandin generation in carrageenan-induced pleurisy by inducible nitric oxide synthase in knockout mice. / Rossi, Antonietta; Cuzzocrea, Salvatore; Mazzon, Emanuela; Serraino, Ivana; De Sarro, Angela; Dugo, Laura; Felice, Maria Rosa; Van de Loo, Fons A J; Di Rosa, Massimo; Musci, Giovanni; Caputi, Achille P.; Sautebin, Lidia.
In: Life Sciences, Vol. 72, No. 10, 24.01.2003, p. 1199-1208.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Regulation of prostaglandin generation in carrageenan-induced pleurisy by inducible nitric oxide synthase in knockout mice
AU - Rossi, Antonietta
AU - Cuzzocrea, Salvatore
AU - Mazzon, Emanuela
AU - Serraino, Ivana
AU - De Sarro, Angela
AU - Dugo, Laura
AU - Felice, Maria Rosa
AU - Van de Loo, Fons A J
AU - Di Rosa, Massimo
AU - Musci, Giovanni
AU - Caputi, Achille P.
AU - Sautebin, Lidia
PY - 2003/1/24
Y1 - 2003/1/24
N2 - In the present study, by comparing the responses in wild-type mice (iNOSWT) and mice lacking (iNOSKO) the inducible (or type 2) nitric oxide synthase (iNOS), we investigated the correlation between endogenous nitric oxide (NO) and prostaglandin (PG) generation in carrageenan-induced pleurisy. The inflammatory response in iNOSKO mice was significantly reduced in respect to iNOSWT animals, as demonstrated by the exudate volume (-63%) and numbers of infiltrating cells (-62%). The levels of NOx in the pleural exudate from carrageenan-treated mice were significantly (p <0.01) decreased in iNOSKO mice (16 ± 7.6 nmoles/mice) compared to iNOSWT animals (133 ± 9 nmoles/mice). Similarly, the amounts of PGE2 in the pleural exudates of carrageenan-treated animals were significantly (p <0.01) lower in iNOSKO compared to iNOSWT mice (120 ± 20 pg/mice vs. 308 ± 51 pg/mice). Also the amounts of 6-keto-PGF1α produced by lungs from carrageenan-treated iNOSKO mice (1.01 ± 0.10 ng/tissue mg) were significantly (p <0.01) reduced compared to iNOSWT carrageenan-treated mice (2.1 ± 0.09 ng/tissue mg). In conclusion our results confirm, by the use of iNOSKO mice that in carrageenan-induced pleurisy NO positively modulates PG biosynthesis.
AB - In the present study, by comparing the responses in wild-type mice (iNOSWT) and mice lacking (iNOSKO) the inducible (or type 2) nitric oxide synthase (iNOS), we investigated the correlation between endogenous nitric oxide (NO) and prostaglandin (PG) generation in carrageenan-induced pleurisy. The inflammatory response in iNOSKO mice was significantly reduced in respect to iNOSWT animals, as demonstrated by the exudate volume (-63%) and numbers of infiltrating cells (-62%). The levels of NOx in the pleural exudate from carrageenan-treated mice were significantly (p <0.01) decreased in iNOSKO mice (16 ± 7.6 nmoles/mice) compared to iNOSWT animals (133 ± 9 nmoles/mice). Similarly, the amounts of PGE2 in the pleural exudates of carrageenan-treated animals were significantly (p <0.01) lower in iNOSKO compared to iNOSWT mice (120 ± 20 pg/mice vs. 308 ± 51 pg/mice). Also the amounts of 6-keto-PGF1α produced by lungs from carrageenan-treated iNOSKO mice (1.01 ± 0.10 ng/tissue mg) were significantly (p <0.01) reduced compared to iNOSWT carrageenan-treated mice (2.1 ± 0.09 ng/tissue mg). In conclusion our results confirm, by the use of iNOSKO mice that in carrageenan-induced pleurisy NO positively modulates PG biosynthesis.
KW - Carrageenan
KW - Cyclooxygenase
KW - Inflammation
KW - Nitric oxide
KW - Prostaglandins
UR - http://www.scopus.com/inward/record.url?scp=0037462289&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037462289&partnerID=8YFLogxK
U2 - 10.1016/S0024-3205(02)02375-5
DO - 10.1016/S0024-3205(02)02375-5
M3 - Article
VL - 72
SP - 1199
EP - 1208
JO - Life Sciences
JF - Life Sciences
SN - 0024-3205
IS - 10
ER -