Regulation of prostaglandin generation in carrageenan-induced pleurisy by inducible nitric oxide synthase in knockout mice

Antonietta Rossi; Salvatore Cuzzocrea; Emanuela Mazzon; Ivana Serraino; Angela De Sarro; Laura Dugo; Maria Rosa Felice; Fons A J Van de Loo; Massimo Di Rosa; Giovanni Musci; Achille P. Caputi; Lidia Sautebin

doi:10.1016/S0024-3205(02)02375-5

Regulation of prostaglandin generation in carrageenan-induced pleurisy by inducible nitric oxide synthase in knockout mice

Antonietta Rossi, Salvatore Cuzzocrea, Emanuela Mazzon, Ivana Serraino, Angela De Sarro, Laura Dugo, Maria Rosa Felice, Fons A J Van de Loo, Massimo Di Rosa, Giovanni Musci, Achille P. Caputi, Lidia Sautebin

IRCCS Centro Neurolesi "Bonino Pulejo"

Research output: Contribution to journal › Article › peer-review

Abstract

In the present study, by comparing the responses in wild-type mice (iNOSWT) and mice lacking (iNOSKO) the inducible (or type 2) nitric oxide synthase (iNOS), we investigated the correlation between endogenous nitric oxide (NO) and prostaglandin (PG) generation in carrageenan-induced pleurisy. The inflammatory response in iNOSKO mice was significantly reduced in respect to iNOSWT animals, as demonstrated by the exudate volume (-63%) and numbers of infiltrating cells (-62%). The levels of NOx in the pleural exudate from carrageenan-treated mice were significantly (p <0.01) decreased in iNOSKO mice (16 ± 7.6 nmoles/mice) compared to iNOSWT animals (133 ± 9 nmoles/mice). Similarly, the amounts of PGE₂ in the pleural exudates of carrageenan-treated animals were significantly (p <0.01) lower in iNOSKO compared to iNOSWT mice (120 ± 20 pg/mice vs. 308 ± 51 pg/mice). Also the amounts of 6-keto-PGF_1α produced by lungs from carrageenan-treated iNOSKO mice (1.01 ± 0.10 ng/tissue mg) were significantly (p <0.01) reduced compared to iNOSWT carrageenan-treated mice (2.1 ± 0.09 ng/tissue mg). In conclusion our results confirm, by the use of iNOSKO mice that in carrageenan-induced pleurisy NO positively modulates PG biosynthesis.

Original language	English
Pages (from-to)	1199-1208
Number of pages	10
Journal	Life Sciences
Volume	72
Issue number	10
DOIs	https://doi.org/10.1016/S0024-3205(02)02375-5
Publication status	Published - Jan 24 2003

Keywords

Carrageenan
Cyclooxygenase
Inflammation
Nitric oxide
Prostaglandins

ASJC Scopus subject areas

Pharmacology

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Rossi, A., Cuzzocrea, S., Mazzon, E., Serraino, I., De Sarro, A., Dugo, L., Felice, M. R., Van de Loo, F. A. J., Di Rosa, M., Musci, G., Caputi, A. P., & Sautebin, L. (2003). Regulation of prostaglandin generation in carrageenan-induced pleurisy by inducible nitric oxide synthase in knockout mice. Life Sciences, 72(10), 1199-1208. https://doi.org/10.1016/S0024-3205(02)02375-5

Regulation of prostaglandin generation in carrageenan-induced pleurisy by inducible nitric oxide synthase in knockout mice. / Rossi, Antonietta; Cuzzocrea, Salvatore; Mazzon, Emanuela; Serraino, Ivana; De Sarro, Angela; Dugo, Laura; Felice, Maria Rosa; Van de Loo, Fons A J; Di Rosa, Massimo; Musci, Giovanni; Caputi, Achille P.; Sautebin, Lidia.

In: Life Sciences, Vol. 72, No. 10, 24.01.2003, p. 1199-1208.

Research output: Contribution to journal › Article › peer-review

Rossi, A, Cuzzocrea, S, Mazzon, E, Serraino, I, De Sarro, A, Dugo, L, Felice, MR, Van de Loo, FAJ, Di Rosa, M, Musci, G, Caputi, AP & Sautebin, L 2003, 'Regulation of prostaglandin generation in carrageenan-induced pleurisy by inducible nitric oxide synthase in knockout mice', Life Sciences, vol. 72, no. 10, pp. 1199-1208. https://doi.org/10.1016/S0024-3205(02)02375-5

Rossi, Antonietta ; Cuzzocrea, Salvatore ; Mazzon, Emanuela ; Serraino, Ivana ; De Sarro, Angela ; Dugo, Laura ; Felice, Maria Rosa ; Van de Loo, Fons A J ; Di Rosa, Massimo ; Musci, Giovanni ; Caputi, Achille P. ; Sautebin, Lidia. / Regulation of prostaglandin generation in carrageenan-induced pleurisy by inducible nitric oxide synthase in knockout mice. In: Life Sciences. 2003 ; Vol. 72, No. 10. pp. 1199-1208.

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title = "Regulation of prostaglandin generation in carrageenan-induced pleurisy by inducible nitric oxide synthase in knockout mice",

abstract = "In the present study, by comparing the responses in wild-type mice (iNOSWT) and mice lacking (iNOSKO) the inducible (or type 2) nitric oxide synthase (iNOS), we investigated the correlation between endogenous nitric oxide (NO) and prostaglandin (PG) generation in carrageenan-induced pleurisy. The inflammatory response in iNOSKO mice was significantly reduced in respect to iNOSWT animals, as demonstrated by the exudate volume (-63%) and numbers of infiltrating cells (-62%). The levels of NOx in the pleural exudate from carrageenan-treated mice were significantly (p <0.01) decreased in iNOSKO mice (16 ± 7.6 nmoles/mice) compared to iNOSWT animals (133 ± 9 nmoles/mice). Similarly, the amounts of PGE2 in the pleural exudates of carrageenan-treated animals were significantly (p <0.01) lower in iNOSKO compared to iNOSWT mice (120 ± 20 pg/mice vs. 308 ± 51 pg/mice). Also the amounts of 6-keto-PGF1α produced by lungs from carrageenan-treated iNOSKO mice (1.01 ± 0.10 ng/tissue mg) were significantly (p <0.01) reduced compared to iNOSWT carrageenan-treated mice (2.1 ± 0.09 ng/tissue mg). In conclusion our results confirm, by the use of iNOSKO mice that in carrageenan-induced pleurisy NO positively modulates PG biosynthesis.",

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author = "Antonietta Rossi and Salvatore Cuzzocrea and Emanuela Mazzon and Ivana Serraino and {De Sarro}, Angela and Laura Dugo and Felice, {Maria Rosa} and {Van de Loo}, {Fons A J} and {Di Rosa}, Massimo and Giovanni Musci and Caputi, {Achille P.} and Lidia Sautebin",

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T1 - Regulation of prostaglandin generation in carrageenan-induced pleurisy by inducible nitric oxide synthase in knockout mice

AU - Rossi, Antonietta

AU - Cuzzocrea, Salvatore

AU - Mazzon, Emanuela

AU - Serraino, Ivana

AU - De Sarro, Angela

AU - Dugo, Laura

AU - Felice, Maria Rosa

AU - Van de Loo, Fons A J

AU - Di Rosa, Massimo

AU - Musci, Giovanni

AU - Caputi, Achille P.

AU - Sautebin, Lidia

PY - 2003/1/24

Y1 - 2003/1/24

N2 - In the present study, by comparing the responses in wild-type mice (iNOSWT) and mice lacking (iNOSKO) the inducible (or type 2) nitric oxide synthase (iNOS), we investigated the correlation between endogenous nitric oxide (NO) and prostaglandin (PG) generation in carrageenan-induced pleurisy. The inflammatory response in iNOSKO mice was significantly reduced in respect to iNOSWT animals, as demonstrated by the exudate volume (-63%) and numbers of infiltrating cells (-62%). The levels of NOx in the pleural exudate from carrageenan-treated mice were significantly (p <0.01) decreased in iNOSKO mice (16 ± 7.6 nmoles/mice) compared to iNOSWT animals (133 ± 9 nmoles/mice). Similarly, the amounts of PGE2 in the pleural exudates of carrageenan-treated animals were significantly (p <0.01) lower in iNOSKO compared to iNOSWT mice (120 ± 20 pg/mice vs. 308 ± 51 pg/mice). Also the amounts of 6-keto-PGF1α produced by lungs from carrageenan-treated iNOSKO mice (1.01 ± 0.10 ng/tissue mg) were significantly (p <0.01) reduced compared to iNOSWT carrageenan-treated mice (2.1 ± 0.09 ng/tissue mg). In conclusion our results confirm, by the use of iNOSKO mice that in carrageenan-induced pleurisy NO positively modulates PG biosynthesis.

AB - In the present study, by comparing the responses in wild-type mice (iNOSWT) and mice lacking (iNOSKO) the inducible (or type 2) nitric oxide synthase (iNOS), we investigated the correlation between endogenous nitric oxide (NO) and prostaglandin (PG) generation in carrageenan-induced pleurisy. The inflammatory response in iNOSKO mice was significantly reduced in respect to iNOSWT animals, as demonstrated by the exudate volume (-63%) and numbers of infiltrating cells (-62%). The levels of NOx in the pleural exudate from carrageenan-treated mice were significantly (p <0.01) decreased in iNOSKO mice (16 ± 7.6 nmoles/mice) compared to iNOSWT animals (133 ± 9 nmoles/mice). Similarly, the amounts of PGE2 in the pleural exudates of carrageenan-treated animals were significantly (p <0.01) lower in iNOSKO compared to iNOSWT mice (120 ± 20 pg/mice vs. 308 ± 51 pg/mice). Also the amounts of 6-keto-PGF1α produced by lungs from carrageenan-treated iNOSKO mice (1.01 ± 0.10 ng/tissue mg) were significantly (p <0.01) reduced compared to iNOSWT carrageenan-treated mice (2.1 ± 0.09 ng/tissue mg). In conclusion our results confirm, by the use of iNOSKO mice that in carrageenan-induced pleurisy NO positively modulates PG biosynthesis.

KW - Carrageenan

KW - Cyclooxygenase

KW - Inflammation

KW - Nitric oxide

KW - Prostaglandins

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