We investigated whether the MusSVts110 gag gene product (P58(gag)) can regulate the novel growth temperature dependence of MuSVts110 RNA splicing. MuSVts110 mutants with either frameshifts or deletions in the gag gene were tested for their ability to maintain the MuSVts110 splicing phenotype. Only small decreases in splicing efficiency and no changes in the thermosensitivity of viral RNA splicing were observed in MuSVts110 gag gene frameshift mutants. Deletions within the gag gene, however, variably decreased MuSVts110 splicing efficiency but had no effect on its thermosensitivity. Another class of MuSVts110 splicing mutants generated by treatment of MuSVts110-infected cells with NiCl2 was also examined. In these 'nickel revertants', P58(gag) is made, but splicing of the viral transcript is nearly complete at all growth temperatures. The splicing of 'tagged' viral RNA transcribed from a modified MuSVts110 DNA introduced into nickel revertant cells remained thermosensitive, arguing against trans effects of viral gene products on splicing efficiency. These experiments indicated that neither the MuSVts110 P58(gag) protein nor any other viral gene product acts in trans to regulate MuSVts110 splicing.
|Number of pages||8|
|Journal||Journal of Virology|
|Publication status||Published - 1990|
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