Regulation of the microtubular cytoskeleton by Polycystin-1 favors focal adhesions turnover to modulate cell adhesion and migration

Maddalena Castelli, Chiara De Pascalis, Gianfranco Distefano, Nadia Ducano, Amanda Oldani, Letizia Lanzetti, Alessandra Boletta

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Polycystin-1 (PC-1) is a large plasma membrane receptor, encoded by the PKD1 gene, which is mutated in most cases of Autosomal Dominant Polycystic Kidney Disease (ADPKD). The disease is characterized by renal cysts. The precise function of PC-1 remains elusive, although several studies suggest that it can regulate the cellular shape in response to external stimuli. We and others reported that PC-1 regulates the actin cytoskeleton and cell migration. Results: Here we show that cells over-expressing PC-1 display enhanced adhesion rates to the substrate, while cells lacking PC-1 have a reduced capability to adhere. In search for the mechanism responsible for this new property of PC-1 we found that this receptor is able to regulate the stability of the microtubules, in addition to its capability to regulate the actin cytoskeleton. The two cytoskeletal components are acting in a coordinated fashion. Notably, we uncovered that PC-1 regulation of the microtubule cytoskeleton impacts on the turnover rates of focal adhesions in migrating cells and we link all these properties to the capability of PC-1 to regulate the activation state of Focal Adhesion Kinase (FAK). Conclusions: In this study we show several new features of the PC-1 receptor in modulating microtubules and adhesion dynamics, which are essential for its capability to regulate migration.

Original languageEnglish
JournalBMC Cell Biology
DOIs
Publication statusAccepted/In press - May 7 2015

Fingerprint

Focal Adhesions
Cytoskeleton
Cell Adhesion
Cell Movement
Microtubules
Actin Cytoskeleton
Autosomal Dominant Polycystic Kidney
polycystic kidney disease 1 protein
Focal Adhesion Protein-Tyrosine Kinases
Cysts
Cell Membrane
Kidney

Keywords

  • Adhesion
  • Focal adhesion kinase
  • Focal adhesion turnover
  • Microtubules
  • Migration
  • Polycystin-1

ASJC Scopus subject areas

  • Cell Biology

Cite this

Regulation of the microtubular cytoskeleton by Polycystin-1 favors focal adhesions turnover to modulate cell adhesion and migration. / Castelli, Maddalena; De Pascalis, Chiara; Distefano, Gianfranco; Ducano, Nadia; Oldani, Amanda; Lanzetti, Letizia; Boletta, Alessandra.

In: BMC Cell Biology, 07.05.2015.

Research output: Contribution to journalArticle

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AU - Castelli, Maddalena

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AU - Distefano, Gianfranco

AU - Ducano, Nadia

AU - Oldani, Amanda

AU - Lanzetti, Letizia

AU - Boletta, Alessandra

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