Regulatory relevance of lipoproteins in interleukin-2 stimulated lymphocytes in vitro

T. Brevini, M. Mantovani, A. Corsini, A. Gambetta, F. Gandolfi, F. Bozzetti, E. Agradi

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Abstract

The regulatory role of very low density lipoproteins (VLDL) in de novo protein synthesis and in natural killer (NK) mediated cytotoxicity against cancer cells was evaluated in IL-2 activated peripheral blood mononuclear cells (PBL). Supplementing of VLDL to the incubation medium induced a potent dose-related inhibitory effect on the synthesis and secretion of (35S) labelled proteins. The maximal inhibitory response was observed at 50 μg/ml cholesterol VLDL. The effect of VLDL on the IL-2 induced ability of lymphocytes to kill cancer cells and to interfere with target cell proliferation was then evaluated. Maximal cytotoxicity was observed at 50μg VLDL/ml as cholesterol. This VLDL-dependent stimulatory effect was associated with a significant decrease in the proliferative effect of the medium conditioned by PBL. The possibility that VLDL mediate the regulation of immune functions by interacting with metabolic patterns expressed by IL-2 stimulated lymphocytes is discussed in light of these results.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalPharmacological Research
Volume27
Issue number1
Publication statusPublished - 1993

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ASJC Scopus subject areas

  • Pharmacology

Cite this

Brevini, T., Mantovani, M., Corsini, A., Gambetta, A., Gandolfi, F., Bozzetti, F., & Agradi, E. (1993). Regulatory relevance of lipoproteins in interleukin-2 stimulated lymphocytes in vitro. Pharmacological Research, 27(1), 45-52.