Relationship between anti-cardiolipin and anti-endothelial cell antibodies in systemic lupus erythematosus

A. Vismara, P. L. Meroni, A. Tincani, E. N. Harris, W. Barcellini, A. Brucato, M. Khamashta, G. R V Hughes, C. Zanussi, G. Balestrieri

Research output: Contribution to journalArticle

Abstract

Anti-endothelial cell antibodies (AECA) have been detected in 51 lupus sera by cell surface radioimmunoassay with a prevalence of 39.2% for IgG and 45.1% for IgM-AECA. No correlations were found between AECA and different clinical or laboratory parameters, including the presence of anti-cardiolipin antibodies and signs associated with the presence of anti-phospholipid antibodies. However, absorption with cardiolipin liposomes partially inhibited endothelial cell binding, and affinity purified anti-cardiolipin antibodies were able to react with intact human endothelial cells. The binding did not occur via Fc receptors since blocking of Fc receptors with rabbit IgG did not affect the endothelial cell reactivity. Taken together our findings support the hypothesis that antibodies directed against negatively charged phospholipids can be part of the anti-endothelial cell antibodies in certain lupus sera. The possible role of these cross-reacting antibodies in the pathogenesis of vascular thrombosis in lupus patients is discussed.

Original languageEnglish
Pages (from-to)247-253
Number of pages7
JournalClinical and Experimental Immunology
Volume74
Issue number2
Publication statusPublished - 1988

Fingerprint

Cardiolipins
Systemic Lupus Erythematosus
Anti-Idiotypic Antibodies
Endothelial Cells
Fc Receptors
Phospholipids
Immunoglobulin G
Antibodies
Serum
Liposomes
Radioimmunoassay
Immunoglobulin M
Blood Vessels
Thrombosis
Rabbits
anti-endothelial cell antibody

ASJC Scopus subject areas

  • Immunology

Cite this

Relationship between anti-cardiolipin and anti-endothelial cell antibodies in systemic lupus erythematosus. / Vismara, A.; Meroni, P. L.; Tincani, A.; Harris, E. N.; Barcellini, W.; Brucato, A.; Khamashta, M.; Hughes, G. R V; Zanussi, C.; Balestrieri, G.

In: Clinical and Experimental Immunology, Vol. 74, No. 2, 1988, p. 247-253.

Research output: Contribution to journalArticle

Vismara, A, Meroni, PL, Tincani, A, Harris, EN, Barcellini, W, Brucato, A, Khamashta, M, Hughes, GRV, Zanussi, C & Balestrieri, G 1988, 'Relationship between anti-cardiolipin and anti-endothelial cell antibodies in systemic lupus erythematosus', Clinical and Experimental Immunology, vol. 74, no. 2, pp. 247-253.
Vismara, A. ; Meroni, P. L. ; Tincani, A. ; Harris, E. N. ; Barcellini, W. ; Brucato, A. ; Khamashta, M. ; Hughes, G. R V ; Zanussi, C. ; Balestrieri, G. / Relationship between anti-cardiolipin and anti-endothelial cell antibodies in systemic lupus erythematosus. In: Clinical and Experimental Immunology. 1988 ; Vol. 74, No. 2. pp. 247-253.
@article{d8b662038ee248288345375bb5de77ce,
title = "Relationship between anti-cardiolipin and anti-endothelial cell antibodies in systemic lupus erythematosus",
abstract = "Anti-endothelial cell antibodies (AECA) have been detected in 51 lupus sera by cell surface radioimmunoassay with a prevalence of 39.2{\%} for IgG and 45.1{\%} for IgM-AECA. No correlations were found between AECA and different clinical or laboratory parameters, including the presence of anti-cardiolipin antibodies and signs associated with the presence of anti-phospholipid antibodies. However, absorption with cardiolipin liposomes partially inhibited endothelial cell binding, and affinity purified anti-cardiolipin antibodies were able to react with intact human endothelial cells. The binding did not occur via Fc receptors since blocking of Fc receptors with rabbit IgG did not affect the endothelial cell reactivity. Taken together our findings support the hypothesis that antibodies directed against negatively charged phospholipids can be part of the anti-endothelial cell antibodies in certain lupus sera. The possible role of these cross-reacting antibodies in the pathogenesis of vascular thrombosis in lupus patients is discussed.",
author = "A. Vismara and Meroni, {P. L.} and A. Tincani and Harris, {E. N.} and W. Barcellini and A. Brucato and M. Khamashta and Hughes, {G. R V} and C. Zanussi and G. Balestrieri",
year = "1988",
language = "English",
volume = "74",
pages = "247--253",
journal = "Clinical and Experimental Immunology",
issn = "0009-9104",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Relationship between anti-cardiolipin and anti-endothelial cell antibodies in systemic lupus erythematosus

AU - Vismara, A.

AU - Meroni, P. L.

AU - Tincani, A.

AU - Harris, E. N.

AU - Barcellini, W.

AU - Brucato, A.

AU - Khamashta, M.

AU - Hughes, G. R V

AU - Zanussi, C.

AU - Balestrieri, G.

PY - 1988

Y1 - 1988

N2 - Anti-endothelial cell antibodies (AECA) have been detected in 51 lupus sera by cell surface radioimmunoassay with a prevalence of 39.2% for IgG and 45.1% for IgM-AECA. No correlations were found between AECA and different clinical or laboratory parameters, including the presence of anti-cardiolipin antibodies and signs associated with the presence of anti-phospholipid antibodies. However, absorption with cardiolipin liposomes partially inhibited endothelial cell binding, and affinity purified anti-cardiolipin antibodies were able to react with intact human endothelial cells. The binding did not occur via Fc receptors since blocking of Fc receptors with rabbit IgG did not affect the endothelial cell reactivity. Taken together our findings support the hypothesis that antibodies directed against negatively charged phospholipids can be part of the anti-endothelial cell antibodies in certain lupus sera. The possible role of these cross-reacting antibodies in the pathogenesis of vascular thrombosis in lupus patients is discussed.

AB - Anti-endothelial cell antibodies (AECA) have been detected in 51 lupus sera by cell surface radioimmunoassay with a prevalence of 39.2% for IgG and 45.1% for IgM-AECA. No correlations were found between AECA and different clinical or laboratory parameters, including the presence of anti-cardiolipin antibodies and signs associated with the presence of anti-phospholipid antibodies. However, absorption with cardiolipin liposomes partially inhibited endothelial cell binding, and affinity purified anti-cardiolipin antibodies were able to react with intact human endothelial cells. The binding did not occur via Fc receptors since blocking of Fc receptors with rabbit IgG did not affect the endothelial cell reactivity. Taken together our findings support the hypothesis that antibodies directed against negatively charged phospholipids can be part of the anti-endothelial cell antibodies in certain lupus sera. The possible role of these cross-reacting antibodies in the pathogenesis of vascular thrombosis in lupus patients is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0023790693&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023790693&partnerID=8YFLogxK

M3 - Article

C2 - 3224443

AN - SCOPUS:0023790693

VL - 74

SP - 247

EP - 253

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

SN - 0009-9104

IS - 2

ER -