Relationship between anti-phospholipid and anti-endothelial cell antibodies III: β2 glycoprotein I mediates the antibody binding to endothelial membranes and induces the expression of adhesion molecules

N. Del Papa, L. Guidali, L. Spatola, P. Bonara, M. O. Borghi, A. Tincani, G. Balestrieri, P. L. Meroni

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To investigate the role of antibodies reacting with β2 glycoprotein I (β2 GPI) in the anti-endothelial cell binding activity present in sera from patients with the anti-phospholipid syndrome. Methods: Sera positive for anti-phospholipid, anti-endothelial and anti-β2 GPI antibodies were studied for their binding activity on endothelial monolayers cultured in the presence or absence of media containing bovine serum as a source of β2 GPI. Anti-endothelial activity was also evaluated on endothelial cells cultured without serum and supplemented with exogenous human purified β2 GPI. Affinity purified anti-β2 GPI antibodies were investigated under the same experimental conditions. Finally, the effect of the incubation of these affinity purified fractions on the expression of adhesion molecules (ELAM-1) was studied. Results: The reactivity of the sera decreased on endothelial cells incubated in serum-free medium, while endothelial cell binding was restored in a dose dependent manner after the addition of exogenous purified human β2 GPI. Affinity purified anti-β2 GPI antibodies obtained from the same sera retained their endothelial cell binding and were able to activate endothelial cells by inducing the ex novo surface expression of adhesion molecules (ELAM-1). Conclusions: These findings indicate that the close association between anti-endothelial and anti-phospholipid antibodies is sustained by antibodies which recognize β2 GPI adhering to the endothelial cells, and can promote their activation.

Original languageEnglish
Pages (from-to)179-185
Number of pages7
JournalClinical and Experimental Rheumatology
Volume13
Issue number2
Publication statusPublished - 1995

Keywords

  • β2-glycoprotein I
  • anti-endothelial antibodies
  • anti-phospholipid syndrome
  • endothelial leukocyte adhesion molecule 1 (ELAM-1)

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

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