Relationship between changes in platelet reactivity and changes in platelet receptor expression induced by physical exercise

Cristina Aurigemma, Andrea Fattorossi, Alfonso Sestito, Gregory A. Sgueglia, Sara Farnetti, Alexia Buzzonetti, Fabio Infusino, Raffaele Landolfi, Giovanni Scambia, Filippo Crea, Gaetano A. Lanza

Research output: Contribution to journalArticle

Abstract

Introduction: In previous studies we have consistently shown a significant increase of platelet reactivity after exercise in patients with obstructive coronary artery disease (CAD). We also observed a significant individual variability in the response to exercise of platelet reactivity in these patients. Whether exercise-induced changes in platelet reactivity correlate with changes in platelet membrane receptors in patients with CAD is unknown. Methods: We studied 26 patients with stable CAD and 10 matched healthy controls who underwent a symptom-limited treadmill exercise stress test. Venous blood samples were collected at rest and within 5 min of peak exercise. Platelet reactivity was measured by the PFA-100 method as time to occlude (closure time, CT) a ring coated with collagen/adenosine diphosphate (C/ADP). Platelet expression of glycoprotein (GP) IIb/IIIa, in both global (CD41) and active form (PAC-1), and P-selectin (CD62P) and formation of leukocyte-platelet aggregates were assessed by flow cytometry. Results: After exercise CT did not change in controls (85.4 ± 12 to 84.0 ± 9 s, p = 0.37), whereas it decreased in CAD patients (98.8 ± 24 to 91.4 ± 25 s, p <0.001). After exercise, CD41 and PAC-1 platelet expression increased significantly in CAD patients (p = 0.04 for both), but not in controls (p = 0.39 and p = 0.98, respectively). To evaluate the relationship between the response to exercise of platelet reactivity and of platelet receptor expression, CAD patients were divided into two groups: CAD group 1 (16 patients, decrease in CT > 5 s after exercise) and CAD group 2 (10 patients no increase in platelet reactivity after exercise). CD41 and PAC-1 expression increased in CAD group 1 (p = 0.008 and p = 0.026, respectively) but not in CAD group 2 (p = 0.39 and p = 0.50, respectively). No significant differences were observed between the 2 groups for changes in CD62P and leukocyte-platelet aggregates. Conclusions: Our data show that, in patients with stable CAD, an increased platelet reactivity to C/ADP stimulation after exercise, as assessed by the PFA-100 method, is specifically associated with an increased expression of platelet GP IIb/IIIa receptor.

Original languageEnglish
Pages (from-to)901-909
Number of pages9
JournalThrombosis Research
Volume120
Issue number6
DOIs
Publication statusPublished - 2007

Fingerprint

Blood Platelets
Coronary Artery Disease
Exercise
Platelet Glycoprotein GPIIb-IIIa Complex
Exercise Test
Adenosine Diphosphate
Leukocytes
Collagen
Integrin beta3
Platelet Membrane Glycoproteins
P-Selectin
Flow Cytometry
Membranes

Keywords

  • Atherosclerosis
  • Exercise stress test
  • Leukocyte-platelet aggregates
  • Platelet
  • Platelet function analyser (PFA)-100

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Relationship between changes in platelet reactivity and changes in platelet receptor expression induced by physical exercise. / Aurigemma, Cristina; Fattorossi, Andrea; Sestito, Alfonso; Sgueglia, Gregory A.; Farnetti, Sara; Buzzonetti, Alexia; Infusino, Fabio; Landolfi, Raffaele; Scambia, Giovanni; Crea, Filippo; Lanza, Gaetano A.

In: Thrombosis Research, Vol. 120, No. 6, 2007, p. 901-909.

Research output: Contribution to journalArticle

Aurigemma, C, Fattorossi, A, Sestito, A, Sgueglia, GA, Farnetti, S, Buzzonetti, A, Infusino, F, Landolfi, R, Scambia, G, Crea, F & Lanza, GA 2007, 'Relationship between changes in platelet reactivity and changes in platelet receptor expression induced by physical exercise', Thrombosis Research, vol. 120, no. 6, pp. 901-909. https://doi.org/10.1016/j.thromres.2007.01.009
Aurigemma, Cristina ; Fattorossi, Andrea ; Sestito, Alfonso ; Sgueglia, Gregory A. ; Farnetti, Sara ; Buzzonetti, Alexia ; Infusino, Fabio ; Landolfi, Raffaele ; Scambia, Giovanni ; Crea, Filippo ; Lanza, Gaetano A. / Relationship between changes in platelet reactivity and changes in platelet receptor expression induced by physical exercise. In: Thrombosis Research. 2007 ; Vol. 120, No. 6. pp. 901-909.
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abstract = "Introduction: In previous studies we have consistently shown a significant increase of platelet reactivity after exercise in patients with obstructive coronary artery disease (CAD). We also observed a significant individual variability in the response to exercise of platelet reactivity in these patients. Whether exercise-induced changes in platelet reactivity correlate with changes in platelet membrane receptors in patients with CAD is unknown. Methods: We studied 26 patients with stable CAD and 10 matched healthy controls who underwent a symptom-limited treadmill exercise stress test. Venous blood samples were collected at rest and within 5 min of peak exercise. Platelet reactivity was measured by the PFA-100 method as time to occlude (closure time, CT) a ring coated with collagen/adenosine diphosphate (C/ADP). Platelet expression of glycoprotein (GP) IIb/IIIa, in both global (CD41) and active form (PAC-1), and P-selectin (CD62P) and formation of leukocyte-platelet aggregates were assessed by flow cytometry. Results: After exercise CT did not change in controls (85.4 ± 12 to 84.0 ± 9 s, p = 0.37), whereas it decreased in CAD patients (98.8 ± 24 to 91.4 ± 25 s, p <0.001). After exercise, CD41 and PAC-1 platelet expression increased significantly in CAD patients (p = 0.04 for both), but not in controls (p = 0.39 and p = 0.98, respectively). To evaluate the relationship between the response to exercise of platelet reactivity and of platelet receptor expression, CAD patients were divided into two groups: CAD group 1 (16 patients, decrease in CT > 5 s after exercise) and CAD group 2 (10 patients no increase in platelet reactivity after exercise). CD41 and PAC-1 expression increased in CAD group 1 (p = 0.008 and p = 0.026, respectively) but not in CAD group 2 (p = 0.39 and p = 0.50, respectively). No significant differences were observed between the 2 groups for changes in CD62P and leukocyte-platelet aggregates. Conclusions: Our data show that, in patients with stable CAD, an increased platelet reactivity to C/ADP stimulation after exercise, as assessed by the PFA-100 method, is specifically associated with an increased expression of platelet GP IIb/IIIa receptor.",
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AU - Aurigemma, Cristina

AU - Fattorossi, Andrea

AU - Sestito, Alfonso

AU - Sgueglia, Gregory A.

AU - Farnetti, Sara

AU - Buzzonetti, Alexia

AU - Infusino, Fabio

AU - Landolfi, Raffaele

AU - Scambia, Giovanni

AU - Crea, Filippo

AU - Lanza, Gaetano A.

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N2 - Introduction: In previous studies we have consistently shown a significant increase of platelet reactivity after exercise in patients with obstructive coronary artery disease (CAD). We also observed a significant individual variability in the response to exercise of platelet reactivity in these patients. Whether exercise-induced changes in platelet reactivity correlate with changes in platelet membrane receptors in patients with CAD is unknown. Methods: We studied 26 patients with stable CAD and 10 matched healthy controls who underwent a symptom-limited treadmill exercise stress test. Venous blood samples were collected at rest and within 5 min of peak exercise. Platelet reactivity was measured by the PFA-100 method as time to occlude (closure time, CT) a ring coated with collagen/adenosine diphosphate (C/ADP). Platelet expression of glycoprotein (GP) IIb/IIIa, in both global (CD41) and active form (PAC-1), and P-selectin (CD62P) and formation of leukocyte-platelet aggregates were assessed by flow cytometry. Results: After exercise CT did not change in controls (85.4 ± 12 to 84.0 ± 9 s, p = 0.37), whereas it decreased in CAD patients (98.8 ± 24 to 91.4 ± 25 s, p <0.001). After exercise, CD41 and PAC-1 platelet expression increased significantly in CAD patients (p = 0.04 for both), but not in controls (p = 0.39 and p = 0.98, respectively). To evaluate the relationship between the response to exercise of platelet reactivity and of platelet receptor expression, CAD patients were divided into two groups: CAD group 1 (16 patients, decrease in CT > 5 s after exercise) and CAD group 2 (10 patients no increase in platelet reactivity after exercise). CD41 and PAC-1 expression increased in CAD group 1 (p = 0.008 and p = 0.026, respectively) but not in CAD group 2 (p = 0.39 and p = 0.50, respectively). No significant differences were observed between the 2 groups for changes in CD62P and leukocyte-platelet aggregates. Conclusions: Our data show that, in patients with stable CAD, an increased platelet reactivity to C/ADP stimulation after exercise, as assessed by the PFA-100 method, is specifically associated with an increased expression of platelet GP IIb/IIIa receptor.

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