Abstract
Introduction: In previous studies we have consistently shown a significant increase of platelet reactivity after exercise in patients with obstructive coronary artery disease (CAD). We also observed a significant individual variability in the response to exercise of platelet reactivity in these patients. Whether exercise-induced changes in platelet reactivity correlate with changes in platelet membrane receptors in patients with CAD is unknown. Methods: We studied 26 patients with stable CAD and 10 matched healthy controls who underwent a symptom-limited treadmill exercise stress test. Venous blood samples were collected at rest and within 5 min of peak exercise. Platelet reactivity was measured by the PFA-100 method as time to occlude (closure time, CT) a ring coated with collagen/adenosine diphosphate (C/ADP). Platelet expression of glycoprotein (GP) IIb/IIIa, in both global (CD41) and active form (PAC-1), and P-selectin (CD62P) and formation of leukocyte-platelet aggregates were assessed by flow cytometry. Results: After exercise CT did not change in controls (85.4 ± 12 to 84.0 ± 9 s, p = 0.37), whereas it decreased in CAD patients (98.8 ± 24 to 91.4 ± 25 s, p <0.001). After exercise, CD41 and PAC-1 platelet expression increased significantly in CAD patients (p = 0.04 for both), but not in controls (p = 0.39 and p = 0.98, respectively). To evaluate the relationship between the response to exercise of platelet reactivity and of platelet receptor expression, CAD patients were divided into two groups: CAD group 1 (16 patients, decrease in CT > 5 s after exercise) and CAD group 2 (10 patients no increase in platelet reactivity after exercise). CD41 and PAC-1 expression increased in CAD group 1 (p = 0.008 and p = 0.026, respectively) but not in CAD group 2 (p = 0.39 and p = 0.50, respectively). No significant differences were observed between the 2 groups for changes in CD62P and leukocyte-platelet aggregates. Conclusions: Our data show that, in patients with stable CAD, an increased platelet reactivity to C/ADP stimulation after exercise, as assessed by the PFA-100 method, is specifically associated with an increased expression of platelet GP IIb/IIIa receptor.
| Original language | English |
|---|---|
| Pages (from-to) | 901-909 |
| Number of pages | 9 |
| Journal | Thrombosis Research |
| Volume | 120 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2007 |
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Keywords
- Atherosclerosis
- Exercise stress test
- Leukocyte-platelet aggregates
- Platelet
- Platelet function analyser (PFA)-100
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
- Hematology
Cite this
Relationship between changes in platelet reactivity and changes in platelet receptor expression induced by physical exercise. / Aurigemma, Cristina; Fattorossi, Andrea; Sestito, Alfonso; Sgueglia, Gregory A.; Farnetti, Sara; Buzzonetti, Alexia; Infusino, Fabio; Landolfi, Raffaele; Scambia, Giovanni; Crea, Filippo; Lanza, Gaetano A.
In: Thrombosis Research, Vol. 120, No. 6, 2007, p. 901-909.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Relationship between changes in platelet reactivity and changes in platelet receptor expression induced by physical exercise
AU - Aurigemma, Cristina
AU - Fattorossi, Andrea
AU - Sestito, Alfonso
AU - Sgueglia, Gregory A.
AU - Farnetti, Sara
AU - Buzzonetti, Alexia
AU - Infusino, Fabio
AU - Landolfi, Raffaele
AU - Scambia, Giovanni
AU - Crea, Filippo
AU - Lanza, Gaetano A.
PY - 2007
Y1 - 2007
N2 - Introduction: In previous studies we have consistently shown a significant increase of platelet reactivity after exercise in patients with obstructive coronary artery disease (CAD). We also observed a significant individual variability in the response to exercise of platelet reactivity in these patients. Whether exercise-induced changes in platelet reactivity correlate with changes in platelet membrane receptors in patients with CAD is unknown. Methods: We studied 26 patients with stable CAD and 10 matched healthy controls who underwent a symptom-limited treadmill exercise stress test. Venous blood samples were collected at rest and within 5 min of peak exercise. Platelet reactivity was measured by the PFA-100 method as time to occlude (closure time, CT) a ring coated with collagen/adenosine diphosphate (C/ADP). Platelet expression of glycoprotein (GP) IIb/IIIa, in both global (CD41) and active form (PAC-1), and P-selectin (CD62P) and formation of leukocyte-platelet aggregates were assessed by flow cytometry. Results: After exercise CT did not change in controls (85.4 ± 12 to 84.0 ± 9 s, p = 0.37), whereas it decreased in CAD patients (98.8 ± 24 to 91.4 ± 25 s, p <0.001). After exercise, CD41 and PAC-1 platelet expression increased significantly in CAD patients (p = 0.04 for both), but not in controls (p = 0.39 and p = 0.98, respectively). To evaluate the relationship between the response to exercise of platelet reactivity and of platelet receptor expression, CAD patients were divided into two groups: CAD group 1 (16 patients, decrease in CT > 5 s after exercise) and CAD group 2 (10 patients no increase in platelet reactivity after exercise). CD41 and PAC-1 expression increased in CAD group 1 (p = 0.008 and p = 0.026, respectively) but not in CAD group 2 (p = 0.39 and p = 0.50, respectively). No significant differences were observed between the 2 groups for changes in CD62P and leukocyte-platelet aggregates. Conclusions: Our data show that, in patients with stable CAD, an increased platelet reactivity to C/ADP stimulation after exercise, as assessed by the PFA-100 method, is specifically associated with an increased expression of platelet GP IIb/IIIa receptor.
AB - Introduction: In previous studies we have consistently shown a significant increase of platelet reactivity after exercise in patients with obstructive coronary artery disease (CAD). We also observed a significant individual variability in the response to exercise of platelet reactivity in these patients. Whether exercise-induced changes in platelet reactivity correlate with changes in platelet membrane receptors in patients with CAD is unknown. Methods: We studied 26 patients with stable CAD and 10 matched healthy controls who underwent a symptom-limited treadmill exercise stress test. Venous blood samples were collected at rest and within 5 min of peak exercise. Platelet reactivity was measured by the PFA-100 method as time to occlude (closure time, CT) a ring coated with collagen/adenosine diphosphate (C/ADP). Platelet expression of glycoprotein (GP) IIb/IIIa, in both global (CD41) and active form (PAC-1), and P-selectin (CD62P) and formation of leukocyte-platelet aggregates were assessed by flow cytometry. Results: After exercise CT did not change in controls (85.4 ± 12 to 84.0 ± 9 s, p = 0.37), whereas it decreased in CAD patients (98.8 ± 24 to 91.4 ± 25 s, p <0.001). After exercise, CD41 and PAC-1 platelet expression increased significantly in CAD patients (p = 0.04 for both), but not in controls (p = 0.39 and p = 0.98, respectively). To evaluate the relationship between the response to exercise of platelet reactivity and of platelet receptor expression, CAD patients were divided into two groups: CAD group 1 (16 patients, decrease in CT > 5 s after exercise) and CAD group 2 (10 patients no increase in platelet reactivity after exercise). CD41 and PAC-1 expression increased in CAD group 1 (p = 0.008 and p = 0.026, respectively) but not in CAD group 2 (p = 0.39 and p = 0.50, respectively). No significant differences were observed between the 2 groups for changes in CD62P and leukocyte-platelet aggregates. Conclusions: Our data show that, in patients with stable CAD, an increased platelet reactivity to C/ADP stimulation after exercise, as assessed by the PFA-100 method, is specifically associated with an increased expression of platelet GP IIb/IIIa receptor.
KW - Atherosclerosis
KW - Exercise stress test
KW - Leukocyte-platelet aggregates
KW - Platelet
KW - Platelet function analyser (PFA)-100
UR - http://www.scopus.com/inward/record.url?scp=34548700839&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34548700839&partnerID=8YFLogxK
U2 - 10.1016/j.thromres.2007.01.009
DO - 10.1016/j.thromres.2007.01.009
M3 - Article
C2 - 17337041
AN - SCOPUS:34548700839
VL - 120
SP - 901
EP - 909
JO - Thrombosis Research
JF - Thrombosis Research
SN - 0049-3848
IS - 6
ER -