TY - JOUR
T1 - Relationship between expression of Fcγ receptors or la antigens and cytolytic activities of alloactivated human T cells
AU - Moretta, Alessandro
AU - Mingari, Maria Cristina
AU - Cerottini, Jean Charles
AU - Fauci, Anthony S.
AU - Moretta, Lorenzo
PY - 1983
Y1 - 1983
N2 - The relationship between expression of Ia antigens or Fcγreceptors (FcγR) and different cytolytic activities of mixed leukocyte culture (MLC)-activated T-cell populations was studied. Lymphocytes mediating specific lysis of target cells bearing the stimulating alloantigens (CTL) could clearly be distinguished from cells mediating antibody-dependent cellular cytotoxicity (ADCC) in that CTL were FcγR- while cells mediating ADCC were FcγR+. In contrast, MLC-generated natural killer (NK) cells were distributed in both the FcγR- and FcγR+ cell fractions. The presence or absence of Ia antigens did not correlate with any of the cytolytic activities studied. In addition, FcγR- and not FcγR+ cells responded in the secondary MLC. Thus, although FcγR+ cells are generated in large numbers in MLC, they do not appear to play a direct role in specific cytotoxicity nor do they proliferate in response to secondary stimulation. However, their ability, following MLC generation, to mediate NK activity and ADCC, both of which may contribute to in vitro and in vivo lysis of allogeneic cells, might explain their appearance following allogeneic stimulation.
AB - The relationship between expression of Ia antigens or Fcγreceptors (FcγR) and different cytolytic activities of mixed leukocyte culture (MLC)-activated T-cell populations was studied. Lymphocytes mediating specific lysis of target cells bearing the stimulating alloantigens (CTL) could clearly be distinguished from cells mediating antibody-dependent cellular cytotoxicity (ADCC) in that CTL were FcγR- while cells mediating ADCC were FcγR+. In contrast, MLC-generated natural killer (NK) cells were distributed in both the FcγR- and FcγR+ cell fractions. The presence or absence of Ia antigens did not correlate with any of the cytolytic activities studied. In addition, FcγR- and not FcγR+ cells responded in the secondary MLC. Thus, although FcγR+ cells are generated in large numbers in MLC, they do not appear to play a direct role in specific cytotoxicity nor do they proliferate in response to secondary stimulation. However, their ability, following MLC generation, to mediate NK activity and ADCC, both of which may contribute to in vitro and in vivo lysis of allogeneic cells, might explain their appearance following allogeneic stimulation.
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U2 - 10.1016/0090-1229(83)90141-1
DO - 10.1016/0090-1229(83)90141-1
M3 - Article
C2 - 6223746
AN - SCOPUS:0020682797
VL - 26
SP - 232
EP - 239
JO - Clinical Immunology and Immunopathology
JF - Clinical Immunology and Immunopathology
SN - 0090-1229
IS - 2
ER -