Relationship between poly(ADP-ribose) polymerase activity and DNA damage induced by zinc dithiocarbamates in mouse and rat liver

L. Scarabelli, P. Giannoni, C. Malfatto, C. Bolognesi, C. F. Cesarone

Research output: Contribution to journalArticlepeer-review

Abstract

The genotoxic effects due to in vivo treatment with zinc dithiocarbamates were evaluated in rat and mouse liver. The two pesticides Zineb and Ziram, belonging to this chemical class, induced an increase in single-strand DNA breaks, as measured by the alkaline elution technique. The nuclear enzyme poly(ADP-ribose) polymerase (pADPRP), a chromatin-bound catalytic protein, utilizing NAD+ as a substrate, was tested by a radiometric procedure. A close relationship between the increased extent of DNA damage and the enhanced level of endogenous pADPRP activity was obtained in rat liver, whereas both parameters remained unchanged in mouse liver.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalMutation Research Letters
Volume302
Issue number1
DOIs
Publication statusPublished - 1993

Keywords

  • DNA damage
  • Liver
  • Pesticides
  • Poly(ADP-ribose) polymerase
  • Zinc dithiocarbamates

ASJC Scopus subject areas

  • Genetics
  • Toxicology

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