TY - JOUR
T1 - Relationship between poly(ADP-ribose) polymerase activity and DNA damage induced by zinc dithiocarbamates in mouse and rat liver
AU - Scarabelli, L.
AU - Giannoni, P.
AU - Malfatto, C.
AU - Bolognesi, C.
AU - Cesarone, C. F.
PY - 1993
Y1 - 1993
N2 - The genotoxic effects due to in vivo treatment with zinc dithiocarbamates were evaluated in rat and mouse liver. The two pesticides Zineb and Ziram, belonging to this chemical class, induced an increase in single-strand DNA breaks, as measured by the alkaline elution technique. The nuclear enzyme poly(ADP-ribose) polymerase (pADPRP), a chromatin-bound catalytic protein, utilizing NAD+ as a substrate, was tested by a radiometric procedure. A close relationship between the increased extent of DNA damage and the enhanced level of endogenous pADPRP activity was obtained in rat liver, whereas both parameters remained unchanged in mouse liver.
AB - The genotoxic effects due to in vivo treatment with zinc dithiocarbamates were evaluated in rat and mouse liver. The two pesticides Zineb and Ziram, belonging to this chemical class, induced an increase in single-strand DNA breaks, as measured by the alkaline elution technique. The nuclear enzyme poly(ADP-ribose) polymerase (pADPRP), a chromatin-bound catalytic protein, utilizing NAD+ as a substrate, was tested by a radiometric procedure. A close relationship between the increased extent of DNA damage and the enhanced level of endogenous pADPRP activity was obtained in rat liver, whereas both parameters remained unchanged in mouse liver.
KW - DNA damage
KW - Liver
KW - Pesticides
KW - Poly(ADP-ribose) polymerase
KW - Zinc dithiocarbamates
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U2 - 10.1016/0165-7992(93)90082-7
DO - 10.1016/0165-7992(93)90082-7
M3 - Article
C2 - 7683100
AN - SCOPUS:0027462807
VL - 302
SP - 1
EP - 6
JO - Mutation Research Letters
JF - Mutation Research Letters
SN - 0165-7992
IS - 1
ER -