Relationship between Rous sarcoma virus-induced expression of membrane antigen and phenotypic transformation

P. M. Comoglio, B. Pani, M. Prat, G. Tarone, C. Monti-Bragadin

Research output: Contribution to journalArticle

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Abstract

Rous sarcoma virus-transformed hamster BHK fibroblasts express a virus-induced cell surface antigen undetectable in cells either transformed by unrelated viruses or infected by transformation-defective strains of Rous sarcoma virus. To clarify whether this antigen plays any role in the process of malignant transformation or is expressed at the cell surface only as a consequence of the acquisition of the transformed phenotype, we have investigated its expression at the cell surface of Rous sarcoma virus-transformed BHK cells treated with dibutyryl cyclic adenosine 3':5'-monophosphate and at the surface of parental BHK cells transiently transformed by the tumor promoter phorbol myristate acetate. In the dibutyryl cyclic adenosine 3':5'-monophosphate-treated cells, in which most of the parameters of the transformed phenotype are reverted to normality, but the product of the transforming gene is still present, virus-induced cell surface antigen is expressed. In the mirror experiment, this antigen is not expressed by phenotypically transformed but genetically normal phorbol myristate acetate-treated cells. It is concluded that the tumor membrane antigen studied is intimately associated with the expression of the function(s) controlled by the transforming gene.

Original languageEnglish
Pages (from-to)4744-4748
Number of pages5
JournalCancer Research
Volume39
Issue number11
Publication statusPublished - 1979

Fingerprint

Rous sarcoma virus
Antigens
Membranes
Tetradecanoylphorbol Acetate
Surface Antigens
Viruses
Oncogenes
Adenosine
Phenotype
Neoplasm Antigens
Cricetinae
Carcinogens
Fibroblasts

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Comoglio, P. M., Pani, B., Prat, M., Tarone, G., & Monti-Bragadin, C. (1979). Relationship between Rous sarcoma virus-induced expression of membrane antigen and phenotypic transformation. Cancer Research, 39(11), 4744-4748.

Relationship between Rous sarcoma virus-induced expression of membrane antigen and phenotypic transformation. / Comoglio, P. M.; Pani, B.; Prat, M.; Tarone, G.; Monti-Bragadin, C.

In: Cancer Research, Vol. 39, No. 11, 1979, p. 4744-4748.

Research output: Contribution to journalArticle

Comoglio, PM, Pani, B, Prat, M, Tarone, G & Monti-Bragadin, C 1979, 'Relationship between Rous sarcoma virus-induced expression of membrane antigen and phenotypic transformation', Cancer Research, vol. 39, no. 11, pp. 4744-4748.
Comoglio PM, Pani B, Prat M, Tarone G, Monti-Bragadin C. Relationship between Rous sarcoma virus-induced expression of membrane antigen and phenotypic transformation. Cancer Research. 1979;39(11):4744-4748.
Comoglio, P. M. ; Pani, B. ; Prat, M. ; Tarone, G. ; Monti-Bragadin, C. / Relationship between Rous sarcoma virus-induced expression of membrane antigen and phenotypic transformation. In: Cancer Research. 1979 ; Vol. 39, No. 11. pp. 4744-4748.
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