Relationship between the two components of the split promoter of eukaryotic tRNA genes

G. Ciliberto, C. Traboni, R. Cortese

Research output: Contribution to journalArticle

Abstract

Plasmids containing eukaryotic tRNA genes are faithfully transcribed in the nucleus of Xenopus laevis oocytes. It has been established that two separated regions within the coding sequence of a tRNA gene are essential and sufficient for promotion of transcription. We have constructed a hybrid tRNA gene containing one essential region from tDNA(sup Leu) and the other from tDNA(sup Pro), both from Caenorhabditis elegans. This hybrid gene is efficiently transcribed, thus showing that the essential regions are independent transcriptional signals regardless of the overall regularities of the structure of tRNA genes. We have also constructed mutants of the tRNA(sup Pro) gene in which the distance between the two essential regions is changed; optimal transcription occurs when this distance is about 40-50 nucleotides.

Original languageEnglish
Pages (from-to)1921-1925
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume79
Issue number6 I
Publication statusPublished - 1982

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Transfer RNA
Genes
RNA, Transfer, Pro
Essential Genes
Caenorhabditis elegans
Xenopus laevis
Oocytes
Plasmids
Nucleotides

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Relationship between the two components of the split promoter of eukaryotic tRNA genes. / Ciliberto, G.; Traboni, C.; Cortese, R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, No. 6 I, 1982, p. 1921-1925.

Research output: Contribution to journalArticle

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