Relationships between paraoxon and 2-coumaranone hydrolytic activities in sera genotyped for PON1 Q192R polymorphism

Roberto Giacominelli-Stuffler, Clotilde Beatrice Angelucci, Laura Liberatoscioli, Claudio Cortese, Enrico Dainese, Mauro Maccarrone

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: To set-up a method for a direct evaluation in human serum of paraoxonase enzymatic activities, establishing a possible correlation with Q192R genotype polymorphism. Design and methods: 101 different human serum samples were genotyped for paraoxonase Q192R polymorphism by PCR restriction analysis, and evaluated spectrophotometrically with regard to paraoxon and 2-coumaranone hydrolytic activities. Both activities of paraoxonase were assayed, quantified through normalization by arylesterase activity, and compared with the data concerning Q/R genetic polymorphism. Results: The mean normalized paraoxonase activity was found to be significantly higher in RR than in QQ human sera (3.99 ± 0.6 versus 1.32 ± 0.44; P <0.0001); instead, the 2-coumaranone hydrolysis showed an opposite trend (0.10 ± 0.02 versus 0.23 ± 0.04, in RR and QQ sera respectively; P <0.0001). Conclusions: These methods were successfully applied to the whole serum, suggesting a possible use of this approach for a clinically relevant phenotypic characterization.

Original languageEnglish
Pages (from-to)1512-1516
Number of pages5
JournalClinical Biochemistry
Volume42
Issue number15
DOIs
Publication statusPublished - Oct 2009

Keywords

  • Enzymatic activities
  • Genetic polymorphism
  • High density lipoproteins
  • Paraoxonase
  • PON1

ASJC Scopus subject areas

  • Clinical Biochemistry

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