Relationships between total CD34+ cells reinfused, CD34+ subsets and engraftment kinetics in breast cancer patients

Claudio Dazzi, Anna Cariello, Giovanni Rosti, Giuseppe Monti, Maura Baioni, Lia Zornetta, Emilia Ferrari, Emanuela Scarpi, Giammaria Fiorentini, Maurizio Marangolo

Research output: Contribution to journalArticlepeer-review

Abstract

Background and Objectives. The aim of the present study was to evaluate the correlation between the number of CD34+ cells transfused and the duration of hypoplasia, and the relationship between various CD34+ subsets (CD34+/33-; CD34+/38-; CD34+/HLA-DR; CD34+/Thy-1+) and engraftment kinetics in a series of patients with breast cancer treated with high doses of thiotepa and melphalan. Design and Methods. We treated 42 consecutive patients: 19 in an adjuvant context (≥4 positive axillary nodes) and 23 for metastatic disease. A combination of thiotepa 600 mg/m2 and melphalan 140- 160 mg/m2 was administered as the conditioning regimen. All patients received peripheral blood progenitor cells (PBPC) and growth factors for hematopoietic rescue. Results. In univariate analysis, we found a significant relationship between the number of CD34+ cells reinfused and the time to hematologic recovery and duration of hospital stay. We observed an inverse correlation between the number of CD34+ cells reinfused and the units of platelets transfused. Cox multivariate analysis confirmed that the number of CD34+ cells reinfused is the most effective predictor of the time to hematologic recovery. CFU-GM resulted to be a better predictor of the duration of hospitalization. Interpretation and Conclusions. We found a significant relationship between the number of PBPC reinfused an the time to hematologic recovery after high doses of thiotepa and melphalan. In our experience, the numbers of subsets of CD34+ cells infused did not give compared additional information to that provided by the total number of CD34+ cells infused. (C) 2000, Ferrata Storti Foundation.

Original languageEnglish
Pages (from-to)396-402
Number of pages7
JournalHaematologica
Volume85
Issue number4
Publication statusPublished - Apr 2000

Keywords

  • Breast cancer
  • CD34 cells subsets
  • Engraftment kinetics
  • High-dose chemotherapy
  • PBPC

ASJC Scopus subject areas

  • Hematology

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