The activity of three human immunodeficiency virus (HIV) protease inhibitors was investigated in human primary monocytes/macrophages (M/M) chronically infected by HIV-1. Saquinavir, KNI272, and ritonavir inhibited the replication of HIV-1 in vitro, with EC550s of ~ 0.5-3.3 μM. However, only partial inhibition was achievable, even at the highest concentrations tested. Also, the activity of these drugs in chronically infected M/M was ~7- to 26-fold lower than in acutely infected M/M and ~2- to 10-fold lower than in chronically infected H9 lymphocytes. When protease inhibitors were removed from cultures of chronically infected M/M, production of virus rapidly returned to the levels found in untreated M/M. Therefore, relatively high concentrations of protease inhibitors are required to suppress HIV-1 production in chronically infected macrophages, and such cells may be a vulnerable point for the escape of virus in patients taking these drugs.
|Number of pages||10|
|Journal||Journal of Infectious Diseases|
|Publication status||Published - 1998|
ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health