Release of diamine oxidase into plasma by glycosaminoglycans in rats

L. D'Agostino, S. Pignata, B. Daniele, R. Ventriglia, G. Ferrari, C. Ferraro, S. Spagnuolo, P. E. Lucchelli, G. Mazzacca

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Plasma diamine oxidase (DAO) values are enhanced by intravenous injection of heparin which releases the enzyme, synthesized in small bowel enterocytes, from binding sites located on endothelial cells of the intestinal microvasculature. Intestinal DAO, in analogy with lipoprotein lipase (another heparin-released enzyme), is believed to be electrostatically linked to endothelial binding sites composed of a glycosaminoglycan (GAG) which is presumably heparan sulphate, but the complete mechanism of enzyme release is not known. In this study we assayed in rats the DAO-releasing capability of heparan sulphate, dermatan sulphate, chondroitin sulphate A and hyaluronic acid, all heparin related compounds. Heparan sulphate, a compound with the same hexosamine as heparin but with a lower concentration of sulphated iduronic acid, induced a very high release of DAO (3-fold less than heparin), while the other tested GAGs, composed of higher proportions of non sulphated uronic acid and with galactosamine instead of glucosamine, induced a significantly lower release. In rats treated with 60 mg heparan sulphate the significant decrease in ileal mucosal DAO activity indicates that, in analogy with heparin, the high plasma enzymatic activity induced is of enterocytic origin. It is suggested that the high charge density of the compounds tested, due to the degree of sulphatation, is the decisive factor in promoting the release of intestinal DAO.

Original languageEnglish
Pages (from-to)228-232
Number of pages5
JournalBBA - General Subjects
Volume993
Issue number2-3
DOIs
Publication statusPublished - Dec 8 1989

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Amine Oxidase (Copper-Containing)
Glycosaminoglycans
Heparin
Rats
Heparitin Sulfate
Plasmas
Enzymes
Iduronic Acid
Binding Sites
Uronic Acids
Hexosamines
Galactosamine
Lipoprotein Lipase
Enterocytes
Chondroitin Sulfates
Glucosamine
Endothelial cells
Hyaluronic Acid
Microvessels
Charge density

Keywords

  • (Rat)
  • Diamine oxidase
  • Glycosaminoglycan
  • Small bowel

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

D'Agostino, L., Pignata, S., Daniele, B., Ventriglia, R., Ferrari, G., Ferraro, C., ... Mazzacca, G. (1989). Release of diamine oxidase into plasma by glycosaminoglycans in rats. BBA - General Subjects, 993(2-3), 228-232. https://doi.org/10.1016/0304-4165(89)90169-4

Release of diamine oxidase into plasma by glycosaminoglycans in rats. / D'Agostino, L.; Pignata, S.; Daniele, B.; Ventriglia, R.; Ferrari, G.; Ferraro, C.; Spagnuolo, S.; Lucchelli, P. E.; Mazzacca, G.

In: BBA - General Subjects, Vol. 993, No. 2-3, 08.12.1989, p. 228-232.

Research output: Contribution to journalArticle

D'Agostino, L, Pignata, S, Daniele, B, Ventriglia, R, Ferrari, G, Ferraro, C, Spagnuolo, S, Lucchelli, PE & Mazzacca, G 1989, 'Release of diamine oxidase into plasma by glycosaminoglycans in rats', BBA - General Subjects, vol. 993, no. 2-3, pp. 228-232. https://doi.org/10.1016/0304-4165(89)90169-4
D'Agostino L, Pignata S, Daniele B, Ventriglia R, Ferrari G, Ferraro C et al. Release of diamine oxidase into plasma by glycosaminoglycans in rats. BBA - General Subjects. 1989 Dec 8;993(2-3):228-232. https://doi.org/10.1016/0304-4165(89)90169-4
D'Agostino, L. ; Pignata, S. ; Daniele, B. ; Ventriglia, R. ; Ferrari, G. ; Ferraro, C. ; Spagnuolo, S. ; Lucchelli, P. E. ; Mazzacca, G. / Release of diamine oxidase into plasma by glycosaminoglycans in rats. In: BBA - General Subjects. 1989 ; Vol. 993, No. 2-3. pp. 228-232.
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