Release of hemopoietic factors by normal human T cell lines with either suppressor or helper activity

L. Lanfrancone, D. Ferrero, E. Gallo, R. Foa, C. Tarella

Research output: Contribution to journalArticlepeer-review

Abstract

We analyzed the release of activities capable of stimulating the in vitro growth of human hemopoietic progenitor cells by long-term cultured T cell growth factor (TCGF)-dependent human T lymphocytes. Seven cell lines tested produced colony-stimulating activity (CSA) as well as burst-promoting activity (BPA). The CSA stimulated primarily the growth of the cells forming colonies after 14 days of incubation. In addition the supernatants from these seven T-cell lines showed the ability to induce the in vitro growth of mixed granulocyte, erythroid, megakaryocyte, macrophage colonies (CFU-GEMM)). The release of hemopoietic factors did not depend on the presence of accessory cells or phytohemagglutinin or serum during the incubation for factor production. In six of the T cell lines the majority of the cells were reactive to the OKT 8 monoclonal antibody (MoAb), whereas one cell line contained mostly OKT 4+ cells. Suppressor activity was detected in three tested OKT 8+ cell lines, while the one OKT 4+ displayed helper activity. All cell lines produced hemopoietic factors with equal efficiency. These results indicate that factors affecting human hematopoiesis are produced by normal T lymphocytes in long-term culture and this property is not related to the helper or suppressor activity of the cultured cells.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalJournal of Cellular Physiology
Volume122
Issue number1
DOIs
Publication statusPublished - 1985

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

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