Release of ICTP and CTX telopeptides from demineralized dentin matrices: Effect of time, mass and surface area

Gianluca Turco, Milena Cadenaro, Tatjana Maravić, Andrea Frassetto, Eleonora Marsich, Annalisa Mazzoni, Roberto Di Lenarda, Franklin R. Tay, David H. Pashley, Lorenzo Breschi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective: The present study evaluated the influence of time, mass and surface area of demineralized dentin collagen matrices on telopeptides release. The hypotheses tested were that the rates of ICTP and CTX release by matrix bound endogenous proteases are 1) not time-dependent, 2) unrelated to specimen mass, 3) unrelated to specimen surface area. Methods: Non-carious human molars (N = 24) were collected and randomly assigned to three groups. Dentin slabs with three different thicknesses: 0.37. mm, 0.75. mm, and 1.50. mm were completely demineralized and stored in artificial saliva for one week. Collagen degradation was evaluated by sampling storage media for ICTP and CTX telopeptidases. Activity of MMPs in the aging medium was evaluated using fluorometric activity assay kit. Results: A statistically significant (p. <. 0.05) decrease in the release of both ICTP and CTX fragments over time was observed irrespective of the specimen thickness. When data were normalized by the specimen mass, no significant differences were observed. Releases of ICTP and CTX were significantly related to the aging time as a function of surface area for the first 12. h. Total MMP activity, mainly related to MMP-2 and -9, decreased with time (p. <. 0.05). Significance: Because the release of collagen fragments was influenced by specimen storage time and surface area, it is likely that cleaved collagen fragments closer to the specimen surface diffuse into the incubation medium; those further away from the exposed surface are still entrapped within the demineralized dentin matrix. Bound MMPs can only degrade the substrate within the limited zone of their molecular mobility.

Original languageEnglish
JournalDental Materials
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Matrix Metalloproteinases
Collagen
Aging of materials
Artificial Saliva
Dentin
Demineralized Dentin Matrix
Assays
Peptide Hydrolases
Sampling
Degradation
Substrates

Keywords

  • Cathepsins
  • Collagen degradation
  • CTX
  • Dentin
  • ICTP
  • Matrix metalloproteinases

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Release of ICTP and CTX telopeptides from demineralized dentin matrices : Effect of time, mass and surface area. / Turco, Gianluca; Cadenaro, Milena; Maravić, Tatjana; Frassetto, Andrea; Marsich, Eleonora; Mazzoni, Annalisa; Di Lenarda, Roberto; Tay, Franklin R.; Pashley, David H.; Breschi, Lorenzo.

In: Dental Materials, 01.01.2018.

Research output: Contribution to journalArticle

Turco, G, Cadenaro, M, Maravić, T, Frassetto, A, Marsich, E, Mazzoni, A, Di Lenarda, R, Tay, FR, Pashley, DH & Breschi, L 2018, 'Release of ICTP and CTX telopeptides from demineralized dentin matrices: Effect of time, mass and surface area', Dental Materials. https://doi.org/10.1016/j.dental.2017.12.003
Turco, Gianluca ; Cadenaro, Milena ; Maravić, Tatjana ; Frassetto, Andrea ; Marsich, Eleonora ; Mazzoni, Annalisa ; Di Lenarda, Roberto ; Tay, Franklin R. ; Pashley, David H. ; Breschi, Lorenzo. / Release of ICTP and CTX telopeptides from demineralized dentin matrices : Effect of time, mass and surface area. In: Dental Materials. 2018.
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abstract = "Objective: The present study evaluated the influence of time, mass and surface area of demineralized dentin collagen matrices on telopeptides release. The hypotheses tested were that the rates of ICTP and CTX release by matrix bound endogenous proteases are 1) not time-dependent, 2) unrelated to specimen mass, 3) unrelated to specimen surface area. Methods: Non-carious human molars (N = 24) were collected and randomly assigned to three groups. Dentin slabs with three different thicknesses: 0.37. mm, 0.75. mm, and 1.50. mm were completely demineralized and stored in artificial saliva for one week. Collagen degradation was evaluated by sampling storage media for ICTP and CTX telopeptidases. Activity of MMPs in the aging medium was evaluated using fluorometric activity assay kit. Results: A statistically significant (p. <. 0.05) decrease in the release of both ICTP and CTX fragments over time was observed irrespective of the specimen thickness. When data were normalized by the specimen mass, no significant differences were observed. Releases of ICTP and CTX were significantly related to the aging time as a function of surface area for the first 12. h. Total MMP activity, mainly related to MMP-2 and -9, decreased with time (p. <. 0.05). Significance: Because the release of collagen fragments was influenced by specimen storage time and surface area, it is likely that cleaved collagen fragments closer to the specimen surface diffuse into the incubation medium; those further away from the exposed surface are still entrapped within the demineralized dentin matrix. Bound MMPs can only degrade the substrate within the limited zone of their molecular mobility.",
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T2 - Effect of time, mass and surface area

AU - Turco, Gianluca

AU - Cadenaro, Milena

AU - Maravić, Tatjana

AU - Frassetto, Andrea

AU - Marsich, Eleonora

AU - Mazzoni, Annalisa

AU - Di Lenarda, Roberto

AU - Tay, Franklin R.

AU - Pashley, David H.

AU - Breschi, Lorenzo

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N2 - Objective: The present study evaluated the influence of time, mass and surface area of demineralized dentin collagen matrices on telopeptides release. The hypotheses tested were that the rates of ICTP and CTX release by matrix bound endogenous proteases are 1) not time-dependent, 2) unrelated to specimen mass, 3) unrelated to specimen surface area. Methods: Non-carious human molars (N = 24) were collected and randomly assigned to three groups. Dentin slabs with three different thicknesses: 0.37. mm, 0.75. mm, and 1.50. mm were completely demineralized and stored in artificial saliva for one week. Collagen degradation was evaluated by sampling storage media for ICTP and CTX telopeptidases. Activity of MMPs in the aging medium was evaluated using fluorometric activity assay kit. Results: A statistically significant (p. <. 0.05) decrease in the release of both ICTP and CTX fragments over time was observed irrespective of the specimen thickness. When data were normalized by the specimen mass, no significant differences were observed. Releases of ICTP and CTX were significantly related to the aging time as a function of surface area for the first 12. h. Total MMP activity, mainly related to MMP-2 and -9, decreased with time (p. <. 0.05). Significance: Because the release of collagen fragments was influenced by specimen storage time and surface area, it is likely that cleaved collagen fragments closer to the specimen surface diffuse into the incubation medium; those further away from the exposed surface are still entrapped within the demineralized dentin matrix. Bound MMPs can only degrade the substrate within the limited zone of their molecular mobility.

AB - Objective: The present study evaluated the influence of time, mass and surface area of demineralized dentin collagen matrices on telopeptides release. The hypotheses tested were that the rates of ICTP and CTX release by matrix bound endogenous proteases are 1) not time-dependent, 2) unrelated to specimen mass, 3) unrelated to specimen surface area. Methods: Non-carious human molars (N = 24) were collected and randomly assigned to three groups. Dentin slabs with three different thicknesses: 0.37. mm, 0.75. mm, and 1.50. mm were completely demineralized and stored in artificial saliva for one week. Collagen degradation was evaluated by sampling storage media for ICTP and CTX telopeptidases. Activity of MMPs in the aging medium was evaluated using fluorometric activity assay kit. Results: A statistically significant (p. <. 0.05) decrease in the release of both ICTP and CTX fragments over time was observed irrespective of the specimen thickness. When data were normalized by the specimen mass, no significant differences were observed. Releases of ICTP and CTX were significantly related to the aging time as a function of surface area for the first 12. h. Total MMP activity, mainly related to MMP-2 and -9, decreased with time (p. <. 0.05). Significance: Because the release of collagen fragments was influenced by specimen storage time and surface area, it is likely that cleaved collagen fragments closer to the specimen surface diffuse into the incubation medium; those further away from the exposed surface are still entrapped within the demineralized dentin matrix. Bound MMPs can only degrade the substrate within the limited zone of their molecular mobility.

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KW - Collagen degradation

KW - CTX

KW - Dentin

KW - ICTP

KW - Matrix metalloproteinases

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