TY - JOUR
T1 - Relevance of class, molecular weight and isoelectric point in predicting human light chain amyloidogenicity
AU - Bellotti, V.
AU - Merlini, G.
AU - Bucciarelli, E.
AU - Perfetti, V.
AU - Quaglini, S.
AU - Ascari, E.
PY - 1990
Y1 - 1990
N2 - The ability to predict the amyloidogenicity of certain light chains may facilitate an earlier diagnosis of AL amyloidosis and, possibly, lead to more effective treatment. Using current methods, available in clinical chemistry laboratories, we assessed the class, the relative molecular mass (M(r)) and the isoelectric point of urinary monoclonal light chains from 35 patients with AL amyloidosis (A+) and 51 without amyloidosis (A-). The light chain class (LCC) was λ in 77% and 45% of A+ and A- patients, respectively. Light chain fragments (LCF) with low M(r) (12-18x103 were detected in the urine of 30/35 A+ patients and in 15/51 A- ones. The mean (SD) isoelectric point (pI) of A+ light chains was 4.8 (1.1) while in A- patients it was 6.2 (1.6). Univariate analysis showed significant differences between the two groups for the three parameters. Discriminant analysis gave a function which allowed a correct allocation of 81% of the cases between the two groups.
AB - The ability to predict the amyloidogenicity of certain light chains may facilitate an earlier diagnosis of AL amyloidosis and, possibly, lead to more effective treatment. Using current methods, available in clinical chemistry laboratories, we assessed the class, the relative molecular mass (M(r)) and the isoelectric point of urinary monoclonal light chains from 35 patients with AL amyloidosis (A+) and 51 without amyloidosis (A-). The light chain class (LCC) was λ in 77% and 45% of A+ and A- patients, respectively. Light chain fragments (LCF) with low M(r) (12-18x103 were detected in the urine of 30/35 A+ patients and in 15/51 A- ones. The mean (SD) isoelectric point (pI) of A+ light chains was 4.8 (1.1) while in A- patients it was 6.2 (1.6). Univariate analysis showed significant differences between the two groups for the three parameters. Discriminant analysis gave a function which allowed a correct allocation of 81% of the cases between the two groups.
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M3 - Article
C2 - 2106912
AN - SCOPUS:0025019313
VL - 74
SP - 65
EP - 69
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 1
ER -