Relevance of hyperglycemia on the timing of functional loss of allogeneic islet transplants: Implication for mouse model

Raffaella Melzi, Manuela Battaglia, Elena Draghici, Ezio Bonifacio, Lorenzo Piemonti

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND. The role of recipient hyperglycemia on timing of allograft survival is unknown. In this study, we investigated if and how variation in recipient glycemia affects the ability to achieve and maintain normoglycemia after transplant of C57BL/6 islets into diabetic BALB/c mice. METHODS AND RESULTS. 85 diabetic BALB/c mice with non-fasting glycaemia ranging between 275 and 600 mg/dL were transplanted with 400 C57BL/6 islets. The time of rejection inversely correlated with the pre-transplant blood glucose concentration (P=0.004). All the 13 mice with normoglycemia beyond 50 days had pretransplant glycemia 100 days function) by the persistence of normoglycemia after allograft removal. The presence of immunosuppression (rapamycin plus FK506 plus anti-IL-2Ra chain mAbs, n=31; rapamycin plus IL-10; n=29) removed the influence of pretransplant hyperglycemia but after treatment withdrawn the timing and the probability of graft loss correlate with the pretransplant hyperglycemia. Pretransplant glycemia was inversely correlated with HOMA-B and serum insulin showing that a significant residual beta cell mass was present in mice with glycemia

Original languageEnglish
Pages (from-to)167-173
Number of pages7
JournalTransplantation
Volume83
Issue number2
DOIs
Publication statusPublished - Jan 2007

Keywords

  • Hyperglycemia
  • Islet transplantation
  • Mouse model

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Fingerprint Dive into the research topics of 'Relevance of hyperglycemia on the timing of functional loss of allogeneic islet transplants: Implication for mouse model'. Together they form a unique fingerprint.

Cite this