Relevance of monoclonal antibodies in the diagnosis of unusual T-cell acute lymphoblastic leukaemia

R. Foa, F. Caligaris Cappio, D. Campana, M. T. Fierro, L. Bergui, M. C. Giubellino, P. Lusso

Research output: Contribution to journalArticle

Abstract

3 Cases of adult acute lymphoblastic leukaemia (ALL), the T-cell nature of which was identified only using a panel of monoclonal antibodies (MoAb), are described. All cases were E-rosette negative, surface immunoglobulin (SmIg) negative, common ALL (CALLA) antigen negative, terminal deoxynucleotidyl transferase (TdT) positive, and acid phosphatase positive. The T-cell origin of the blasts was demonstrated by the positivity with RFA-1, a MoAb which detects an antigen of MW-65-69,000 present on the membrane of thymocytes and mature T-lymphocytes. In addition, 2 of the 3 cases were positive with OKT6, which recognizes cortical thymocytes. MoAb directed against more mature T lineage cells (OKT3, OKT4, OKT8, OKT11A) were consistently negative (≤12%). These findings indicate that the use of a combination of MoAb is important in detecting individual cases of T-ALL, which otherwise might be classified as undifferentiated acute leukaemia or null-ALL. MoAb detecting T-cell antigenic determinant of MW 65-69,000 (e.g. RFA-1, OKT1, Leu1) appear the most specific reagents for T-ALL.

Original languageEnglish
Pages (from-to)303-307
Number of pages5
JournalScandinavian Journal of Haematology
Volume30
Issue number4
Publication statusPublished - 1983

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Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
Monoclonal Antibodies
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Neprilysin
Thymocytes
T-Lymphocytes
Muromonab-CD3
B-Cell Antigen Receptors
DNA Nucleotidylexotransferase
Acid Phosphatase
Epitopes
Leukemia
Antigens
Membranes

ASJC Scopus subject areas

  • Hematology

Cite this

Foa, R., Caligaris Cappio, F., Campana, D., Fierro, M. T., Bergui, L., Giubellino, M. C., & Lusso, P. (1983). Relevance of monoclonal antibodies in the diagnosis of unusual T-cell acute lymphoblastic leukaemia. Scandinavian Journal of Haematology, 30(4), 303-307.

Relevance of monoclonal antibodies in the diagnosis of unusual T-cell acute lymphoblastic leukaemia. / Foa, R.; Caligaris Cappio, F.; Campana, D.; Fierro, M. T.; Bergui, L.; Giubellino, M. C.; Lusso, P.

In: Scandinavian Journal of Haematology, Vol. 30, No. 4, 1983, p. 303-307.

Research output: Contribution to journalArticle

Foa, R, Caligaris Cappio, F, Campana, D, Fierro, MT, Bergui, L, Giubellino, MC & Lusso, P 1983, 'Relevance of monoclonal antibodies in the diagnosis of unusual T-cell acute lymphoblastic leukaemia', Scandinavian Journal of Haematology, vol. 30, no. 4, pp. 303-307.
Foa R, Caligaris Cappio F, Campana D, Fierro MT, Bergui L, Giubellino MC et al. Relevance of monoclonal antibodies in the diagnosis of unusual T-cell acute lymphoblastic leukaemia. Scandinavian Journal of Haematology. 1983;30(4):303-307.
Foa, R. ; Caligaris Cappio, F. ; Campana, D. ; Fierro, M. T. ; Bergui, L. ; Giubellino, M. C. ; Lusso, P. / Relevance of monoclonal antibodies in the diagnosis of unusual T-cell acute lymphoblastic leukaemia. In: Scandinavian Journal of Haematology. 1983 ; Vol. 30, No. 4. pp. 303-307.
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AB - 3 Cases of adult acute lymphoblastic leukaemia (ALL), the T-cell nature of which was identified only using a panel of monoclonal antibodies (MoAb), are described. All cases were E-rosette negative, surface immunoglobulin (SmIg) negative, common ALL (CALLA) antigen negative, terminal deoxynucleotidyl transferase (TdT) positive, and acid phosphatase positive. The T-cell origin of the blasts was demonstrated by the positivity with RFA-1, a MoAb which detects an antigen of MW-65-69,000 present on the membrane of thymocytes and mature T-lymphocytes. In addition, 2 of the 3 cases were positive with OKT6, which recognizes cortical thymocytes. MoAb directed against more mature T lineage cells (OKT3, OKT4, OKT8, OKT11A) were consistently negative (≤12%). These findings indicate that the use of a combination of MoAb is important in detecting individual cases of T-ALL, which otherwise might be classified as undifferentiated acute leukaemia or null-ALL. MoAb detecting T-cell antigenic determinant of MW 65-69,000 (e.g. RFA-1, OKT1, Leu1) appear the most specific reagents for T-ALL.

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