Abstract
3 Cases of adult acute lymphoblastic leukaemia (ALL), the T-cell nature of which was identified only using a panel of monoclonal antibodies (MoAb), are described. All cases were E-rosette negative, surface immunoglobulin (SmIg) negative, common ALL (CALLA) antigen negative, terminal deoxynucleotidyl transferase (TdT) positive, and acid phosphatase positive. The T-cell origin of the blasts was demonstrated by the positivity with RFA-1, a MoAb which detects an antigen of MW-65-69,000 present on the membrane of thymocytes and mature T-lymphocytes. In addition, 2 of the 3 cases were positive with OKT6, which recognizes cortical thymocytes. MoAb directed against more mature T lineage cells (OKT3, OKT4, OKT8, OKT11A) were consistently negative (≤12%). These findings indicate that the use of a combination of MoAb is important in detecting individual cases of T-ALL, which otherwise might be classified as undifferentiated acute leukaemia or null-ALL. MoAb detecting T-cell antigenic determinant of MW 65-69,000 (e.g. RFA-1, OKT1, Leu1) appear the most specific reagents for T-ALL.
| Original language | English |
|---|---|
| Pages (from-to) | 303-307 |
| Number of pages | 5 |
| Journal | Scandinavian Journal of Haematology |
| Volume | 30 |
| Issue number | 4 |
| Publication status | Published - 1983 |
Fingerprint
ASJC Scopus subject areas
- Hematology
Cite this
Relevance of monoclonal antibodies in the diagnosis of unusual T-cell acute lymphoblastic leukaemia. / Foa, R.; Caligaris Cappio, F.; Campana, D.; Fierro, M. T.; Bergui, L.; Giubellino, M. C.; Lusso, P.
In: Scandinavian Journal of Haematology, Vol. 30, No. 4, 1983, p. 303-307.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Relevance of monoclonal antibodies in the diagnosis of unusual T-cell acute lymphoblastic leukaemia
AU - Foa, R.
AU - Caligaris Cappio, F.
AU - Campana, D.
AU - Fierro, M. T.
AU - Bergui, L.
AU - Giubellino, M. C.
AU - Lusso, P.
PY - 1983
Y1 - 1983
N2 - 3 Cases of adult acute lymphoblastic leukaemia (ALL), the T-cell nature of which was identified only using a panel of monoclonal antibodies (MoAb), are described. All cases were E-rosette negative, surface immunoglobulin (SmIg) negative, common ALL (CALLA) antigen negative, terminal deoxynucleotidyl transferase (TdT) positive, and acid phosphatase positive. The T-cell origin of the blasts was demonstrated by the positivity with RFA-1, a MoAb which detects an antigen of MW-65-69,000 present on the membrane of thymocytes and mature T-lymphocytes. In addition, 2 of the 3 cases were positive with OKT6, which recognizes cortical thymocytes. MoAb directed against more mature T lineage cells (OKT3, OKT4, OKT8, OKT11A) were consistently negative (≤12%). These findings indicate that the use of a combination of MoAb is important in detecting individual cases of T-ALL, which otherwise might be classified as undifferentiated acute leukaemia or null-ALL. MoAb detecting T-cell antigenic determinant of MW 65-69,000 (e.g. RFA-1, OKT1, Leu1) appear the most specific reagents for T-ALL.
AB - 3 Cases of adult acute lymphoblastic leukaemia (ALL), the T-cell nature of which was identified only using a panel of monoclonal antibodies (MoAb), are described. All cases were E-rosette negative, surface immunoglobulin (SmIg) negative, common ALL (CALLA) antigen negative, terminal deoxynucleotidyl transferase (TdT) positive, and acid phosphatase positive. The T-cell origin of the blasts was demonstrated by the positivity with RFA-1, a MoAb which detects an antigen of MW-65-69,000 present on the membrane of thymocytes and mature T-lymphocytes. In addition, 2 of the 3 cases were positive with OKT6, which recognizes cortical thymocytes. MoAb directed against more mature T lineage cells (OKT3, OKT4, OKT8, OKT11A) were consistently negative (≤12%). These findings indicate that the use of a combination of MoAb is important in detecting individual cases of T-ALL, which otherwise might be classified as undifferentiated acute leukaemia or null-ALL. MoAb detecting T-cell antigenic determinant of MW 65-69,000 (e.g. RFA-1, OKT1, Leu1) appear the most specific reagents for T-ALL.
UR - http://www.scopus.com/inward/record.url?scp=0020535952&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020535952&partnerID=8YFLogxK
M3 - Article
C2 - 6190215
AN - SCOPUS:0020535952
VL - 30
SP - 303
EP - 307
JO - European Journal of Haematology
JF - European Journal of Haematology
SN - 0902-4441
IS - 4
ER -