Reliability of 3H-thymidine labeling index: cell proliferation of colorectal carcinoma in three different centers.

M. Ponz de Leon, A. Costa, G. Lanza, A. Scalmati, L. Roncucci, A. Faranda, C. Di Gregorio, R. Fante, G. Colella, R. Silvestrini

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Although the 3H-thymidine labeling index (TLI) has been used extensively as a biologic marker of increased susceptibility to neoplasms or as a prognostic indicator of the clinical outcome in cancer patients, there is still some concern regarding its accuracy and reliability as a marker of cell replication. A study on the prognostic value of TLI in colorectal cancer gave us the ability to evaluate how such a measurement may vary in different laboratories. A total of 150 malignant tumors of the large bowel were studied in the period from 1988 to 1989. Microautoradiography was carried out in tumor fragments taken at surgery. There were only slight differences among the three centers involved in the investigation, mainly as regards the culture medium, exposure time, and the addition of O2 + CO2 during the incubation. No significant difference was observed among TLI values recorded in the three centers (18.3 +/- 0.9; 17.4 +/- 0.9; 15.7 +/- 1.0 mean +/- SE), and the ranges of values were almost identical. Similarly, the numbers of total and of labeled cells counted for each patient were comparable among the three centers. The frequency distribution of TLI showed a peak value between 10 and 19.9% in all three centers. Moreover, more than 80% of the observed values were within the range of 10 to 29.9%. In addition, the neoplastic areas with the highest proliferative activity ("high" TLI) showed a frequency distribution once again rather similar among the three centers.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish
Pages (from-to)627-631
Number of pages5
JournalModern Pathology
Volume4
Issue number5
Publication statusPublished - Sep 1991

Fingerprint

Thymidine
Colorectal Neoplasms
Cell Proliferation
Neoplasms
Culture Media
Biomarkers

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Ponz de Leon, M., Costa, A., Lanza, G., Scalmati, A., Roncucci, L., Faranda, A., ... Silvestrini, R. (1991). Reliability of 3H-thymidine labeling index: cell proliferation of colorectal carcinoma in three different centers. Modern Pathology, 4(5), 627-631.

Reliability of 3H-thymidine labeling index : cell proliferation of colorectal carcinoma in three different centers. / Ponz de Leon, M.; Costa, A.; Lanza, G.; Scalmati, A.; Roncucci, L.; Faranda, A.; Di Gregorio, C.; Fante, R.; Colella, G.; Silvestrini, R.

In: Modern Pathology, Vol. 4, No. 5, 09.1991, p. 627-631.

Research output: Contribution to journalArticle

Ponz de Leon, M, Costa, A, Lanza, G, Scalmati, A, Roncucci, L, Faranda, A, Di Gregorio, C, Fante, R, Colella, G & Silvestrini, R 1991, 'Reliability of 3H-thymidine labeling index: cell proliferation of colorectal carcinoma in three different centers.', Modern Pathology, vol. 4, no. 5, pp. 627-631.
Ponz de Leon M, Costa A, Lanza G, Scalmati A, Roncucci L, Faranda A et al. Reliability of 3H-thymidine labeling index: cell proliferation of colorectal carcinoma in three different centers. Modern Pathology. 1991 Sep;4(5):627-631.
Ponz de Leon, M. ; Costa, A. ; Lanza, G. ; Scalmati, A. ; Roncucci, L. ; Faranda, A. ; Di Gregorio, C. ; Fante, R. ; Colella, G. ; Silvestrini, R. / Reliability of 3H-thymidine labeling index : cell proliferation of colorectal carcinoma in three different centers. In: Modern Pathology. 1991 ; Vol. 4, No. 5. pp. 627-631.
@article{c040ab895bbe49d9bbc77590b916d5ff,
title = "Reliability of 3H-thymidine labeling index: cell proliferation of colorectal carcinoma in three different centers.",
abstract = "Although the 3H-thymidine labeling index (TLI) has been used extensively as a biologic marker of increased susceptibility to neoplasms or as a prognostic indicator of the clinical outcome in cancer patients, there is still some concern regarding its accuracy and reliability as a marker of cell replication. A study on the prognostic value of TLI in colorectal cancer gave us the ability to evaluate how such a measurement may vary in different laboratories. A total of 150 malignant tumors of the large bowel were studied in the period from 1988 to 1989. Microautoradiography was carried out in tumor fragments taken at surgery. There were only slight differences among the three centers involved in the investigation, mainly as regards the culture medium, exposure time, and the addition of O2 + CO2 during the incubation. No significant difference was observed among TLI values recorded in the three centers (18.3 +/- 0.9; 17.4 +/- 0.9; 15.7 +/- 1.0 mean +/- SE), and the ranges of values were almost identical. Similarly, the numbers of total and of labeled cells counted for each patient were comparable among the three centers. The frequency distribution of TLI showed a peak value between 10 and 19.9{\%} in all three centers. Moreover, more than 80{\%} of the observed values were within the range of 10 to 29.9{\%}. In addition, the neoplastic areas with the highest proliferative activity ({"}high{"} TLI) showed a frequency distribution once again rather similar among the three centers.(ABSTRACT TRUNCATED AT 250 WORDS)",
author = "{Ponz de Leon}, M. and A. Costa and G. Lanza and A. Scalmati and L. Roncucci and A. Faranda and {Di Gregorio}, C. and R. Fante and G. Colella and R. Silvestrini",
year = "1991",
month = "9",
language = "English",
volume = "4",
pages = "627--631",
journal = "Modern Pathology",
issn = "0893-3952",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Reliability of 3H-thymidine labeling index

T2 - cell proliferation of colorectal carcinoma in three different centers.

AU - Ponz de Leon, M.

AU - Costa, A.

AU - Lanza, G.

AU - Scalmati, A.

AU - Roncucci, L.

AU - Faranda, A.

AU - Di Gregorio, C.

AU - Fante, R.

AU - Colella, G.

AU - Silvestrini, R.

PY - 1991/9

Y1 - 1991/9

N2 - Although the 3H-thymidine labeling index (TLI) has been used extensively as a biologic marker of increased susceptibility to neoplasms or as a prognostic indicator of the clinical outcome in cancer patients, there is still some concern regarding its accuracy and reliability as a marker of cell replication. A study on the prognostic value of TLI in colorectal cancer gave us the ability to evaluate how such a measurement may vary in different laboratories. A total of 150 malignant tumors of the large bowel were studied in the period from 1988 to 1989. Microautoradiography was carried out in tumor fragments taken at surgery. There were only slight differences among the three centers involved in the investigation, mainly as regards the culture medium, exposure time, and the addition of O2 + CO2 during the incubation. No significant difference was observed among TLI values recorded in the three centers (18.3 +/- 0.9; 17.4 +/- 0.9; 15.7 +/- 1.0 mean +/- SE), and the ranges of values were almost identical. Similarly, the numbers of total and of labeled cells counted for each patient were comparable among the three centers. The frequency distribution of TLI showed a peak value between 10 and 19.9% in all three centers. Moreover, more than 80% of the observed values were within the range of 10 to 29.9%. In addition, the neoplastic areas with the highest proliferative activity ("high" TLI) showed a frequency distribution once again rather similar among the three centers.(ABSTRACT TRUNCATED AT 250 WORDS)

AB - Although the 3H-thymidine labeling index (TLI) has been used extensively as a biologic marker of increased susceptibility to neoplasms or as a prognostic indicator of the clinical outcome in cancer patients, there is still some concern regarding its accuracy and reliability as a marker of cell replication. A study on the prognostic value of TLI in colorectal cancer gave us the ability to evaluate how such a measurement may vary in different laboratories. A total of 150 malignant tumors of the large bowel were studied in the period from 1988 to 1989. Microautoradiography was carried out in tumor fragments taken at surgery. There were only slight differences among the three centers involved in the investigation, mainly as regards the culture medium, exposure time, and the addition of O2 + CO2 during the incubation. No significant difference was observed among TLI values recorded in the three centers (18.3 +/- 0.9; 17.4 +/- 0.9; 15.7 +/- 1.0 mean +/- SE), and the ranges of values were almost identical. Similarly, the numbers of total and of labeled cells counted for each patient were comparable among the three centers. The frequency distribution of TLI showed a peak value between 10 and 19.9% in all three centers. Moreover, more than 80% of the observed values were within the range of 10 to 29.9%. In addition, the neoplastic areas with the highest proliferative activity ("high" TLI) showed a frequency distribution once again rather similar among the three centers.(ABSTRACT TRUNCATED AT 250 WORDS)

UR - http://www.scopus.com/inward/record.url?scp=0026217983&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026217983&partnerID=8YFLogxK

M3 - Article

C2 - 1758875

AN - SCOPUS:0026217983

VL - 4

SP - 627

EP - 631

JO - Modern Pathology

JF - Modern Pathology

SN - 0893-3952

IS - 5

ER -