Reliability of alpha-naphthyl-acetate esterase staining of blood smears for the enumeration of circulating human T lymphocytes

F. A. Ferrari, R. Maccario, M. Marconi, M. A. Vitiello, A. G. Ugazio, V. Burgio, A. G. Siccardi

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Abstract

Cytocentrifuged preparations of glutaraldehyde-fixed E rosettes were stained for acid alpha-naphthyl-acetate esterase activity (ANAE). On average, 84% of the rosette-forming cells (E +) also stained positively for the enzyme activity (A +). ANAE staining of blood smears of thirty-three healthy controls was compared to E rosetting by a conventional method. A + and E + fractional values are highly significantly correlated; the regression of E + on A + is E + fractional values are highly significantly correlated; the regression of E + on A + is E + = 0.805.A + + 16.8 (P <10 -6). Since the correlation between E + and A + cells is both statistically and cytologically significant, we propose blood smear ANAE staining as a simple and convenient method to enumerate circulating T lymphocytes in clinical practice.

Original languageEnglish
Pages (from-to)358-362
Number of pages5
JournalClinical and Experimental Immunology
Volume41
Issue number2
Publication statusPublished - 1980

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ASJC Scopus subject areas

  • Immunology

Cite this

Ferrari, F. A., Maccario, R., Marconi, M., Vitiello, M. A., Ugazio, A. G., Burgio, V., & Siccardi, A. G. (1980). Reliability of alpha-naphthyl-acetate esterase staining of blood smears for the enumeration of circulating human T lymphocytes. Clinical and Experimental Immunology, 41(2), 358-362.