Cytocentrifuged preparations of glutaraldehyde-fixed E rosettes were stained for acid alpha-naphthyl-acetate esterase activity (ANAE). On average, 84% of the rosette-forming cells (E +) also stained positively for the enzyme activity (A +). ANAE staining of blood smears of thirty-three healthy controls was compared to E rosetting by a conventional method. A + and E + fractional values are highly significantly correlated; the regression of E + on A + is E + fractional values are highly significantly correlated; the regression of E + on A + is E + = 0.805.A + + 16.8 (P <10 -6). Since the correlation between E + and A + cells is both statistically and cytologically significant, we propose blood smear ANAE staining as a simple and convenient method to enumerate circulating T lymphocytes in clinical practice.
|Number of pages||5|
|Journal||Clinical and Experimental Immunology|
|Publication status||Published - 1980|
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