Reliability of an in vitro short-term assay to predict the drug sensitivity of human breast cancer

M. G. Daidone, R. Silvestrini, O. Sanfilippo, N. Zaffaroni, M. Varini, M. De Lena

Research output: Contribution to journalArticlepeer-review

Abstract

The feasibility and reliability of an in vitro assay that evaluates drug interference on nucleic acid precursor incorporation were investigated on 135 previously untreated locally advanced breast cancers. The assay, which was carried out on tumor fragments incubated for 3 hours with drugs, proved to be feasible on a sufficiently high percentage of biopsy specimens (70%) for routine clinical use. In vitro drug activity evaluated with this assay appeared to reproduce the clinical patterns of sensitivity of the tumor type as well as of the individual tumors. In fact, in vitro response rates to conventional agents resembled the clinical response rates reported for the same agents used in monochemotherapy. From a retrospective-correlative study carried out on 41 patients treated in vitro and in vivo with the same drugs (Adriamycin [doxorubicin] and vincristine), in vitro effect of Adriamycin on 3H-uridine incorporation appeared significantly correlated with clinical response (overall agreement, 78%; P = 0.0032) with specific agreements of sensitivity and resistance of 75% and 81%, respectively.

Original languageEnglish
Pages (from-to)450-456
Number of pages7
JournalCancer
Volume56
Issue number3
DOIs
Publication statusPublished - 1985

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Fingerprint

Dive into the research topics of 'Reliability of an in vitro short-term assay to predict the drug sensitivity of human breast cancer'. Together they form a unique fingerprint.

Cite this