Reliability of radioactive inulin as a marker of glomerular filtration rate in the rat

Vittorio E. Andreucci; Antonio Dal Canton; Ariberto Corradi; Carla Gherardi

doi:10.1007/BF00592643

Reliability of radioactive inulin as a marker of glomerular filtration rate in the rat

Vittorio E. Andreucci, Antonio Dal Canton, Ariberto Corradi, Carla Gherardi

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article › peer-review

Abstract

Clearance experiments were carried out in rats for evaluating the reliability of Inulin-Methoxy-H³ as marker for GFR determination. When H³ Inulin clearance was compared with C¹⁴ Polyethylen Glycol (PEG) 1000 clearance, a linear relationship was observed (y=0.021+0.867 x;r=0.98). H³ Inulin clearance/C¹⁴ PEG clearance ratio was 0.88±0.09 (S.D.), i.e. a value no different from those reported in literature for unlabeled Inulin, C¹⁴ Inulin and Polyfructosan S. Several types of scintillation solution were tested to evaluate the stability with time of count rate of radioactive Inulin. A perfect reproducibility with time was observed in the clearance values in the kidney as a whole (GFR) and in single nephrons (SNGFR) with the following scintillators: Triton X 100-Toluene-PPO, Aquasol and Instagel. Useful suggestions on the way of using labeled Inulin are given.

Original language	English
Pages (from-to)	347-358
Number of pages	12
Journal	Pflugers Archiv European Journal of Physiology
Volume	350
Issue number	4
DOIs	https://doi.org/10.1007/BF00592643
Publication status	Published - Dec 1974

Keywords

C Polyethylen Glycol 1000
Inulin-Carboxyl-C
Inulin-Methoxy-H
Scintillation Solution
Single Nephron Glomerular Filtration Rate

ASJC Scopus subject areas

Physiology

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10.1007/BF00592643

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title = "Reliability of radioactive inulin as a marker of glomerular filtration rate in the rat",

abstract = "Clearance experiments were carried out in rats for evaluating the reliability of Inulin-Methoxy-H3 as marker for GFR determination. When H3 Inulin clearance was compared with C14 Polyethylen Glycol (PEG) 1000 clearance, a linear relationship was observed (y=0.021+0.867 x;r=0.98). H3 Inulin clearance/C14 PEG clearance ratio was 0.88±0.09 (S.D.), i.e. a value no different from those reported in literature for unlabeled Inulin, C14 Inulin and Polyfructosan S. Several types of scintillation solution were tested to evaluate the stability with time of count rate of radioactive Inulin. A perfect reproducibility with time was observed in the clearance values in the kidney as a whole (GFR) and in single nephrons (SNGFR) with the following scintillators: Triton X 100-Toluene-PPO, Aquasol and Instagel. Useful suggestions on the way of using labeled Inulin are given.",

keywords = "C Polyethylen Glycol 1000, Inulin-Carboxyl-C, Inulin-Methoxy-H, Scintillation Solution, Single Nephron Glomerular Filtration Rate",

author = "Andreucci, {Vittorio E.} and Canton, {Antonio Dal} and Ariberto Corradi and Carla Gherardi",

year = "1974",

month = dec,

doi = "10.1007/BF00592643",

language = "English",

volume = "350",

pages = "347--358",

journal = "Pflugers Archiv European Journal of Physiology",

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T1 - Reliability of radioactive inulin as a marker of glomerular filtration rate in the rat

AU - Andreucci, Vittorio E.

AU - Canton, Antonio Dal

AU - Corradi, Ariberto

AU - Gherardi, Carla

PY - 1974/12

Y1 - 1974/12

N2 - Clearance experiments were carried out in rats for evaluating the reliability of Inulin-Methoxy-H3 as marker for GFR determination. When H3 Inulin clearance was compared with C14 Polyethylen Glycol (PEG) 1000 clearance, a linear relationship was observed (y=0.021+0.867 x;r=0.98). H3 Inulin clearance/C14 PEG clearance ratio was 0.88±0.09 (S.D.), i.e. a value no different from those reported in literature for unlabeled Inulin, C14 Inulin and Polyfructosan S. Several types of scintillation solution were tested to evaluate the stability with time of count rate of radioactive Inulin. A perfect reproducibility with time was observed in the clearance values in the kidney as a whole (GFR) and in single nephrons (SNGFR) with the following scintillators: Triton X 100-Toluene-PPO, Aquasol and Instagel. Useful suggestions on the way of using labeled Inulin are given.

AB - Clearance experiments were carried out in rats for evaluating the reliability of Inulin-Methoxy-H3 as marker for GFR determination. When H3 Inulin clearance was compared with C14 Polyethylen Glycol (PEG) 1000 clearance, a linear relationship was observed (y=0.021+0.867 x;r=0.98). H3 Inulin clearance/C14 PEG clearance ratio was 0.88±0.09 (S.D.), i.e. a value no different from those reported in literature for unlabeled Inulin, C14 Inulin and Polyfructosan S. Several types of scintillation solution were tested to evaluate the stability with time of count rate of radioactive Inulin. A perfect reproducibility with time was observed in the clearance values in the kidney as a whole (GFR) and in single nephrons (SNGFR) with the following scintillators: Triton X 100-Toluene-PPO, Aquasol and Instagel. Useful suggestions on the way of using labeled Inulin are given.

KW - C Polyethylen Glycol 1000

KW - Inulin-Carboxyl-C

KW - Inulin-Methoxy-H

KW - Scintillation Solution

KW - Single Nephron Glomerular Filtration Rate

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ER -

Reliability of radioactive inulin as a marker of glomerular filtration rate in the rat

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Keywords

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