Abstract
Transcriptional repression occurs during meiotic maturation of Xenopus oocytes. Injection of a DNA template containing an hsp70 promoter into Xenopus oocytes, followed by progesterone-induced maturation has been used to demonstrate a dynamic competition between the assembly of transcription factor-containing nucleoprotein complexes and repressive nucleosomal arrays during the maturation process. In particular, it is shown that increased levels of injected heat shock protein, the transcriptional activator Gal4-VP16 or the DNA template itself all lead to reduced repression of transcription on maturation. Conversely, injection of additional histone increases repression. Repression of transcription is shown to be accompanied by the formation of a more regular array of nucleosomes and by an increase in the efficiency of nucleosome assembly on the injected plasmid. Meiotic maturation is therefore accompanied by replacement of transcription factor complexes by a repressive chromatin environment.
| Original language | English |
|---|---|
| Pages (from-to) | 4361-4373 |
| Number of pages | 13 |
| Journal | EMBO Journal |
| Volume | 16 |
| Issue number | 14 |
| DOIs | |
| Publication status | Published - Jul 16 1997 |
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Keywords
- Heat shock
- Meiosis
- Nucleoprotein
- Transcription repression
- Xenopus
ASJC Scopus subject areas
- Cell Biology
- Genetics
Cite this
Remodeling of regulatory nucleoprotein complexes on the Xenopus hsp70 promoter during meiotic maturation of the Xenopus oocyte. / Landsberger, Nicoletta; Wolffe, Alan P.
In: EMBO Journal, Vol. 16, No. 14, 16.07.1997, p. 4361-4373.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Remodeling of regulatory nucleoprotein complexes on the Xenopus hsp70 promoter during meiotic maturation of the Xenopus oocyte
AU - Landsberger, Nicoletta
AU - Wolffe, Alan P.
PY - 1997/7/16
Y1 - 1997/7/16
N2 - Transcriptional repression occurs during meiotic maturation of Xenopus oocytes. Injection of a DNA template containing an hsp70 promoter into Xenopus oocytes, followed by progesterone-induced maturation has been used to demonstrate a dynamic competition between the assembly of transcription factor-containing nucleoprotein complexes and repressive nucleosomal arrays during the maturation process. In particular, it is shown that increased levels of injected heat shock protein, the transcriptional activator Gal4-VP16 or the DNA template itself all lead to reduced repression of transcription on maturation. Conversely, injection of additional histone increases repression. Repression of transcription is shown to be accompanied by the formation of a more regular array of nucleosomes and by an increase in the efficiency of nucleosome assembly on the injected plasmid. Meiotic maturation is therefore accompanied by replacement of transcription factor complexes by a repressive chromatin environment.
AB - Transcriptional repression occurs during meiotic maturation of Xenopus oocytes. Injection of a DNA template containing an hsp70 promoter into Xenopus oocytes, followed by progesterone-induced maturation has been used to demonstrate a dynamic competition between the assembly of transcription factor-containing nucleoprotein complexes and repressive nucleosomal arrays during the maturation process. In particular, it is shown that increased levels of injected heat shock protein, the transcriptional activator Gal4-VP16 or the DNA template itself all lead to reduced repression of transcription on maturation. Conversely, injection of additional histone increases repression. Repression of transcription is shown to be accompanied by the formation of a more regular array of nucleosomes and by an increase in the efficiency of nucleosome assembly on the injected plasmid. Meiotic maturation is therefore accompanied by replacement of transcription factor complexes by a repressive chromatin environment.
KW - Heat shock
KW - Meiosis
KW - Nucleoprotein
KW - Transcription repression
KW - Xenopus
UR - http://www.scopus.com/inward/record.url?scp=0030739851&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030739851&partnerID=8YFLogxK
U2 - 10.1093/emboj/16.14.4361
DO - 10.1093/emboj/16.14.4361
M3 - Article
VL - 16
SP - 4361
EP - 4373
JO - EMBO Journal
JF - EMBO Journal
SN - 0261-4189
IS - 14
ER -