Remodelling of the nuclear lamina during human cytomegalovirus infection: Role of the viral proteins pUL50 and pUL53

Daria Camozzi, Sara Pignatelli, Cecilia Valvo, Giovanna Lattanzi, Cristina Capanni, Paola Dal Monte, Maria Paola Landini

Research output: Contribution to journalArticle

Abstract

A fundamental step in the efficient production of human cytomegalovirus (HCMV) progeny is viral egress from the nucleus to the cytoplasm of infected cells. In the family Herpesviridae, this process involves alteration of nuclear lamina components by two highly conserved proteins, whose homologues in HCMV are named pUL50 and pUL53. This study showed that HCMV infection induced the mislocalization of nuclear lamins and that pUL50 and pUL53 play a role in this event. At late stages of infection, both lamin A/C and lamin B showed an irregular distribution on the nuclear rim, coincident with areas of pUL53 accumulation. No variations in the total amount of nuclear lamins could be detected, supporting the view that HCMV induces a qualitative, rather than a quantitative, alteration of these cellular components, as has been suggested previously for other herpesviruses. Interestingly, pUL53, in the absence of other viral products, localized diffusely in the nucleus, whilst the co-expression and interaction of pUL53 with its partner, pUL50, restored its nuclear rim localization in distinct patches, thus indicating that pUL50 is sufficient to induce the localization of pUL53 observed during virus infection. Importantly, analysis of the nuclear lamina in the presence of pUL50-pUL53 complexes at the nuclear boundary and in the absence of other viral products showed that the two viral proteins were sufficient to promote alterations of lamins, strongly resembling those observed during HCMV infection. These results suggest that pUL50 and pUL53 may play an important role in the exit of virions from the nucleus by inducing structural modifications of the nuclear lamina.

Original languageEnglish
Pages (from-to)731-740
Number of pages10
JournalJournal of General Virology
Volume89
Issue number3
DOIs
Publication statusPublished - Mar 2008

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Nuclear Lamina
Cytomegalovirus Infections
Viral Proteins
Lamins
Cytomegalovirus
Herpesviridae
Lamin Type B
Lamin Type A
Virus Diseases
Virion
Cytoplasm
Infection
Proteins

ASJC Scopus subject areas

  • Virology
  • Immunology

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Remodelling of the nuclear lamina during human cytomegalovirus infection : Role of the viral proteins pUL50 and pUL53. / Camozzi, Daria; Pignatelli, Sara; Valvo, Cecilia; Lattanzi, Giovanna; Capanni, Cristina; Dal Monte, Paola; Landini, Maria Paola.

In: Journal of General Virology, Vol. 89, No. 3, 03.2008, p. 731-740.

Research output: Contribution to journalArticle

Camozzi, Daria ; Pignatelli, Sara ; Valvo, Cecilia ; Lattanzi, Giovanna ; Capanni, Cristina ; Dal Monte, Paola ; Landini, Maria Paola. / Remodelling of the nuclear lamina during human cytomegalovirus infection : Role of the viral proteins pUL50 and pUL53. In: Journal of General Virology. 2008 ; Vol. 89, No. 3. pp. 731-740.
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