Removal of domain D2 or D3 of the human urokinase receptor does not affect ligand affinity

Leena Riittinen, Paola Limongi, Massimo P. Crippa, Massimo Conese, Luciano Hernandez-Marrero, Francesca Fazioli, Francesco Blasi

Research output: Contribution to journalArticle

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Abstract

The main ligand-binding determinant of the human urokinase receptor (uPAR) is located in the amino terminal domain D1, but when isolated this domain presents a 1500 fold lower affinity than the intact three-domain uPAR (D1D2D3). uPAR mutants missing either domain 2 (D1HD3) or domain 3 (D1D2) were expressed in murine LB6 cells and showed to be properly GPI-anchored to the cell surface. Binding assays with [ 125I]ATF demonstrated that these mutants possessed a normal (D1D2) or slightly reduced (D1HD3) affinity, indicating that a high ligand-affinity may be achieved by a combination of D1 with domain D2 or D3.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalFEBS Letters
Volume381
Issue number1-2
DOIs
Publication statusPublished - Feb 26 1996

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Urokinase-Type Plasminogen Activator
Ligands
Assays

Keywords

  • GPI-anchored protein
  • uPA
  • Urokinase receptor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Riittinen, L., Limongi, P., Crippa, M. P., Conese, M., Hernandez-Marrero, L., Fazioli, F., & Blasi, F. (1996). Removal of domain D2 or D3 of the human urokinase receptor does not affect ligand affinity. FEBS Letters, 381(1-2), 1-6. https://doi.org/10.1016/0014-5793(96)00033-6

Removal of domain D2 or D3 of the human urokinase receptor does not affect ligand affinity. / Riittinen, Leena; Limongi, Paola; Crippa, Massimo P.; Conese, Massimo; Hernandez-Marrero, Luciano; Fazioli, Francesca; Blasi, Francesco.

In: FEBS Letters, Vol. 381, No. 1-2, 26.02.1996, p. 1-6.

Research output: Contribution to journalArticle

Riittinen, L, Limongi, P, Crippa, MP, Conese, M, Hernandez-Marrero, L, Fazioli, F & Blasi, F 1996, 'Removal of domain D2 or D3 of the human urokinase receptor does not affect ligand affinity', FEBS Letters, vol. 381, no. 1-2, pp. 1-6. https://doi.org/10.1016/0014-5793(96)00033-6
Riittinen, Leena ; Limongi, Paola ; Crippa, Massimo P. ; Conese, Massimo ; Hernandez-Marrero, Luciano ; Fazioli, Francesca ; Blasi, Francesco. / Removal of domain D2 or D3 of the human urokinase receptor does not affect ligand affinity. In: FEBS Letters. 1996 ; Vol. 381, No. 1-2. pp. 1-6.
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