Removal of oligomers and n-mers from the immobiline chemicals for isoelectric focusing

Thierry Rabilloud, Jean Jacques Pernelle, Juan Pedro Wahrmann, Cecilia Gelfi, Pier Giorgio Righetti

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Three different methods are described for removal of unwanted polymerization products from Immobilines: (a) adsorption onto silica columns; (b) passage through reversed-phase cartridges and (c) use of polymeric adsorbents. The best method is the use of the XAD-2 polymeric adsorbent, as it gives the highest yield of purified monomer (ca. 90%) coupled with the highest removal of polymeric material (ca. 95%). It is demonstrated that the removal of oligomers and n-mers from Immobilines is achieved not by gel filtration, but by an hydrophobic adsorption mechanism onto the surface of the polymeric adsorbent.

Original languageEnglish
Pages (from-to)105-113
Number of pages9
JournalJournal of Chromatography A
Volume402
Issue numberC
DOIs
Publication statusPublished - 1987

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Isoelectric Focusing
Oligomers
Adsorbents
Adsorption
Polymerization
Silicon Dioxide
Gel Chromatography
Monomers
Gels
Polymers
Immobiline

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Removal of oligomers and n-mers from the immobiline chemicals for isoelectric focusing. / Rabilloud, Thierry; Pernelle, Jean Jacques; Wahrmann, Juan Pedro; Gelfi, Cecilia; Righetti, Pier Giorgio.

In: Journal of Chromatography A, Vol. 402, No. C, 1987, p. 105-113.

Research output: Contribution to journalArticle

Rabilloud, Thierry ; Pernelle, Jean Jacques ; Wahrmann, Juan Pedro ; Gelfi, Cecilia ; Righetti, Pier Giorgio. / Removal of oligomers and n-mers from the immobiline chemicals for isoelectric focusing. In: Journal of Chromatography A. 1987 ; Vol. 402, No. C. pp. 105-113.
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