Removal of oligomers and n-mers from the immobiline chemicals for isoelectric focusing

Thierry Rabilloud, Jean Jacques Pernelle, Juan Pedro Wahrmann, Cecilia Gelfi, Pier Giorgio Righetti

Research output: Contribution to journalArticle

Abstract

Three different methods are described for removal of unwanted polymerization products from Immobilines: (a) adsorption onto silica columns; (b) passage through reversed-phase cartridges and (c) use of polymeric adsorbents. The best method is the use of the XAD-2 polymeric adsorbent, as it gives the highest yield of purified monomer (ca. 90%) coupled with the highest removal of polymeric material (ca. 95%). It is demonstrated that the removal of oligomers and n-mers from Immobilines is achieved not by gel filtration, but by an hydrophobic adsorption mechanism onto the surface of the polymeric adsorbent.

Original languageEnglish
Pages (from-to)105-113
Number of pages9
JournalJournal of Chromatography A
Volume402
Issue numberC
DOIs
Publication statusPublished - 1987

ASJC Scopus subject areas

  • Analytical Chemistry

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