Three different methods are described for removal of unwanted polymerization products from Immobilines: (a) adsorption onto silica columns; (b) passage through reversed-phase cartridges and (c) use of polymeric adsorbents. The best method is the use of the XAD-2 polymeric adsorbent, as it gives the highest yield of purified monomer (ca. 90%) coupled with the highest removal of polymeric material (ca. 95%). It is demonstrated that the removal of oligomers and n-mers from Immobilines is achieved not by gel filtration, but by an hydrophobic adsorption mechanism onto the surface of the polymeric adsorbent.
ASJC Scopus subject areas
- Analytical Chemistry