Renal cell carcinoma-induced immunosuppression: An immunophenotypic study of lymphocyte subpopulations and circulating dendritic cells

Camillo Porta, Lucia Bonomi, Beatrice Lillaz, Chiara Paglino, Bianca Rovati, Ilaria Imarisio, Patrizia Morbini, Chiara Villa, Marco Danova, Mario Mensi, Bruno Rovereto

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Abstract

Background: Renal cell carcinoma (RCC)-induced immune dysfunction in patients at first diagnosis was investigated. Patients and Methods: The main circulating lymphocyte subsets, the total number of circulating and intratumor dendritic cells and the titers of circulating VEGF were quantified in 47 RCC patients, using flow cytometric, immunohistochemical and ELISA assays. Results: Despite a significant activation of CD3/HLA-DR + lymphocytes and of the CD56 + NK subset, RCC patients presented a marked immunosuppression of CD4/CD45RA naïve T-cells, CD4/CD45RO memory T-cells, CD16 + NK-cells, and total circulating dendritic cells, as well as a significant increase of lymphocytes co-expressing the CD4 and CD8 antigens. Finally, CD16 +/CD56 + NK and DCs were poorly represented in tumor specimens. Conclusion: The complex immunological dysfunctions demonstrated involve different levels of immunocompetence and indicate a pattern of major disturbance of the immune system.

Original languageEnglish
Pages (from-to)165-174
Number of pages10
JournalAnticancer Research
Volume27
Issue number1 A
Publication statusPublished - Jan 2007

Fingerprint

Lymphocyte Subsets
Renal Cell Carcinoma
Immunosuppression
Dendritic Cells
Lymphocytes
CD8 Antigens
T-Lymphocytes
Immunocompetence
CD4 Antigens
HLA-DR Antigens
Natural Killer Cells
Vascular Endothelial Growth Factor A
Immune System
Enzyme-Linked Immunosorbent Assay
Neoplasms

Keywords

  • Dendritic cells
  • Immunosuppression
  • Lymphocytic subpopulations
  • Renal cell carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Renal cell carcinoma-induced immunosuppression : An immunophenotypic study of lymphocyte subpopulations and circulating dendritic cells. / Porta, Camillo; Bonomi, Lucia; Lillaz, Beatrice; Paglino, Chiara; Rovati, Bianca; Imarisio, Ilaria; Morbini, Patrizia; Villa, Chiara; Danova, Marco; Mensi, Mario; Rovereto, Bruno.

In: Anticancer Research, Vol. 27, No. 1 A, 01.2007, p. 165-174.

Research output: Contribution to journalArticle

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AU - Porta, Camillo

AU - Bonomi, Lucia

AU - Lillaz, Beatrice

AU - Paglino, Chiara

AU - Rovati, Bianca

AU - Imarisio, Ilaria

AU - Morbini, Patrizia

AU - Villa, Chiara

AU - Danova, Marco

AU - Mensi, Mario

AU - Rovereto, Bruno

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N2 - Background: Renal cell carcinoma (RCC)-induced immune dysfunction in patients at first diagnosis was investigated. Patients and Methods: The main circulating lymphocyte subsets, the total number of circulating and intratumor dendritic cells and the titers of circulating VEGF were quantified in 47 RCC patients, using flow cytometric, immunohistochemical and ELISA assays. Results: Despite a significant activation of CD3/HLA-DR + lymphocytes and of the CD56 + NK subset, RCC patients presented a marked immunosuppression of CD4/CD45RA naïve T-cells, CD4/CD45RO memory T-cells, CD16 + NK-cells, and total circulating dendritic cells, as well as a significant increase of lymphocytes co-expressing the CD4 and CD8 antigens. Finally, CD16 +/CD56 + NK and DCs were poorly represented in tumor specimens. Conclusion: The complex immunological dysfunctions demonstrated involve different levels of immunocompetence and indicate a pattern of major disturbance of the immune system.

AB - Background: Renal cell carcinoma (RCC)-induced immune dysfunction in patients at first diagnosis was investigated. Patients and Methods: The main circulating lymphocyte subsets, the total number of circulating and intratumor dendritic cells and the titers of circulating VEGF were quantified in 47 RCC patients, using flow cytometric, immunohistochemical and ELISA assays. Results: Despite a significant activation of CD3/HLA-DR + lymphocytes and of the CD56 + NK subset, RCC patients presented a marked immunosuppression of CD4/CD45RA naïve T-cells, CD4/CD45RO memory T-cells, CD16 + NK-cells, and total circulating dendritic cells, as well as a significant increase of lymphocytes co-expressing the CD4 and CD8 antigens. Finally, CD16 +/CD56 + NK and DCs were poorly represented in tumor specimens. Conclusion: The complex immunological dysfunctions demonstrated involve different levels of immunocompetence and indicate a pattern of major disturbance of the immune system.

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