Renal cyclophilin-like protein gene expression parallels changes in sodium excretion in experimental nephrosis and is positively modulated by atrial natriuretic peptide

Silvia Orisio, Norberto Perico, Luca Benatti, Lorena Longaretti, Susanna Amuchastegui, Giuseppe Remuzzi

Research output: Contribution to journalArticlepeer-review

Abstract

Experimental evidence is available to indicate that intrarenal mechanisms play a role in the impaired salt excretion of nephrotic syndrome by multiple and still uncompletely defined mediators. It is documented herein that the gene encoding for cyclophilin-like protein (Cy-LP) is up-regulated in renal medulla from adriamycin (ADR)-treated rats as compared with control animals. In the cortex of rats with ADR nephrosis, no change in Cy-LP as compared with that in controls was found for the entire observation period. By contrast, in the medulla of nephrotic rats, Cy-LP gene expression was significantly higher than in controls. Values of urinary Na excretion were inversely correlated to Cy-LP mRNA expression levels. Because in ADR nephrosis a blunted natriuretic response to ANP has been previously reported, it was investigated whether ANP infusion modulated Cy-LP mRNA in the renal medulla. ADR-treated rats, but not control rats, infused for 1 h with ANP (1 μg/kg·min) had a significant (P <0.05) increase in medullary Cy-LP mRNA as compared with nephrotic animals receiving the vehicle alone. These findings might be taken to suggest that renal Cy-LP gene expression is positively modulated in nephrotic syndrome and parallels changes in sodium excretion.

Original languageEnglish
Pages (from-to)1710-1716
Number of pages7
JournalJournal of the American Society of Nephrology
Volume3
Issue number10
Publication statusPublished - Apr 1993

Keywords

  • Atrial natriuretic peptide
  • Cyclophilin-like protein
  • Experimental nephrosis

ASJC Scopus subject areas

  • Nephrology

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