Renal expression of monocyte chemoattractant protein-1 in lupus autoimmune mice

Carla Zoja, Xue Hui Liu, Roberta Donadelli, Mauro Abbate, Davide Testa, Daniela Corna, Giulia Taraboletti, Annunciata Vecchi, Qiang Gang Dong, Barrett J. Rollins, Tullio Bertani, Giuseppe Remuzzi

Research output: Contribution to journalArticle

Abstract

Mononuclear cell infiltration in glomeruli and renal interstitium is a prominent feature of some types of glomerulonephritis, including lupus nephritis. The mechanism(s) underlying monocyte influx into the kidney is not fully understood. Recently, monocyte chemoattractant protein-1 (MCP-1) has been identified as a chemotactic factor involved in the recruitment of monocytes/macrophages in the glomeruli of rats with mesangioproliferative as well as anti-glomerular basement membrane glomerulonephritis. In the study presented here, renal MCP-1 mRNA expression in New Zealand Black x New Zealand White (NZB/W) F1 mice, a model of genetically determined immune complex disease that mimics systemic lupus in humans, was investigated. Northern blot analysis revealed a single 0.7 kb MCP-1 transcript of very low intensity in kidneys from 2-month-old NZB/W mice that had not yet developed proteinuria nor renal damage. Message levels, which increased markedly with the progression of nephritis and in association with mononuclear cell infiltration, were 10- and 15- fold higher in 8-10-month-old mice than in 2-month-old mice. By in situ hybridization, increased expression of MCP-1 mRNA was demonstrated in glomeruli and, even more striking, in tubular epithelial cells. Western blot analysis demonstrated increased expression of MCP-1 protein in kidneys of 10-month-old NZB/W mice, consistent with MCP-1 mRNA data. When NZB/W mice were treated with cyclophosphamide up to 12 months of age, expression of MCP-1 in the renal tissue remained low, the influx of inflammatory cells did not appear, and glomerular and tubular structures remained well preserved. These data suggest that elevated MCP-1 might act as a signal for inflammatory cells to infiltrate the kidney in lupus nephritis.

Original languageEnglish
Pages (from-to)720-729
Number of pages10
JournalJournal of the American Society of Nephrology
Volume8
Issue number5
Publication statusPublished - May 1997

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Chemokine CCL2
Kidney
Inbred NZB Mouse
Lupus Nephritis
Glomerulonephritis
New Zealand
Messenger RNA
Monocytes
Immune Complex Diseases
Glomerular Basement Membrane
Nephritis
Chemotactic Factors
Proteinuria
Northern Blotting
Cyclophosphamide
In Situ Hybridization
Western Blotting
Epithelial Cells
Macrophages

ASJC Scopus subject areas

  • Nephrology

Cite this

Renal expression of monocyte chemoattractant protein-1 in lupus autoimmune mice. / Zoja, Carla; Liu, Xue Hui; Donadelli, Roberta; Abbate, Mauro; Testa, Davide; Corna, Daniela; Taraboletti, Giulia; Vecchi, Annunciata; Dong, Qiang Gang; Rollins, Barrett J.; Bertani, Tullio; Remuzzi, Giuseppe.

In: Journal of the American Society of Nephrology, Vol. 8, No. 5, 05.1997, p. 720-729.

Research output: Contribution to journalArticle

Zoja, C, Liu, XH, Donadelli, R, Abbate, M, Testa, D, Corna, D, Taraboletti, G, Vecchi, A, Dong, QG, Rollins, BJ, Bertani, T & Remuzzi, G 1997, 'Renal expression of monocyte chemoattractant protein-1 in lupus autoimmune mice', Journal of the American Society of Nephrology, vol. 8, no. 5, pp. 720-729.
Zoja, Carla ; Liu, Xue Hui ; Donadelli, Roberta ; Abbate, Mauro ; Testa, Davide ; Corna, Daniela ; Taraboletti, Giulia ; Vecchi, Annunciata ; Dong, Qiang Gang ; Rollins, Barrett J. ; Bertani, Tullio ; Remuzzi, Giuseppe. / Renal expression of monocyte chemoattractant protein-1 in lupus autoimmune mice. In: Journal of the American Society of Nephrology. 1997 ; Vol. 8, No. 5. pp. 720-729.
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