Renin modulates HIV replication in T cells

Nirupama Chandel, Kamesh Ayasolla, Xiqian Lan, Partab Rai, Joanna Mikulak, Mohammad Husain, Ashwani Malhotra, Joseph McGowan, Pravin C. Singhal

Research output: Contribution to journalArticle

Abstract

HIV is known to subvert cellular machinery to enhance its replication. Recently, HIV has been reported to enhance TC renin expression. We hypothesized that HIV induces and maintains high renin expression to promote its own replication in TCs. Renin enhanced HIV replication in TCs in a dose-dependent manner. (P)RR-deficient TCs, as well as those lacking renin, displayed attenuated NF-κB activity and HIV replication. TCs treated with renin and Hpr displayed activation of the (P)RR-PLZF protein signaling cascade. Renin, HIV, and Hpr activated the PI3K pathway. Both renin and Hpr cleaved Agt (a renin substrate) to Ang I and also cleaved Gag polyproteins (protease substrate) to p24. Furthermore, aliskiren, a renin inhibitor, reduced renin- and Hpr-induced cleavage of Agt and Gag polyproteins. These findings indicate that renin contributes to HIV replication in TCs via the (P)RR-PLZF signaling cascade and through cleavage of the Gag polyproteins.

Original languageEnglish
Pages (from-to)601-609
Number of pages9
JournalJournal of Leukocyte Biology
Volume96
Issue number4
DOIs
Publication statusPublished - Oct 1 2014

Keywords

  • Aspartyl protease
  • Vitamin D receptors

ASJC Scopus subject areas

  • Cell Biology
  • Immunology
  • Medicine(all)

Fingerprint Dive into the research topics of 'Renin modulates HIV replication in T cells'. Together they form a unique fingerprint.

  • Cite this

    Chandel, N., Ayasolla, K., Lan, X., Rai, P., Mikulak, J., Husain, M., Malhotra, A., McGowan, J., & Singhal, P. C. (2014). Renin modulates HIV replication in T cells. Journal of Leukocyte Biology, 96(4), 601-609. https://doi.org/10.1189/JLB.2A0414-192R