Abstract
8-oxo-7,8-dihydroguanine (8-oxoG) is a potent mutagenic lesion that forms at elevated levels in cellular DNA and is repaired with low efficiency in human cells. Unlike its human counterpart, the Drosophila S3 ribosomal/repair protein is endowed with a vigorous 8 oxoG repair activity that is associated to β,δ-elimination AP lyase activity. We have recently observed that pure GST-tagged Drosophila S3 protein can significantly accelerate the in vitro repair of 8 oxoG performed by human and mouse cell extracts [Cappelli et al., unpublished data]. In this work, we have transfected Chinese hamster cells with mammalian expression vectors containing the Drosophila S3 cDNA. The cells synthesized both S3 mRNA and protein but no improved repair of 8 oxoguanine was observed. Factors important for the proper expression of Drosophila genes in mammalian cells are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 113-121 |
| Number of pages | 9 |
| Journal | Teratogenesis Carcinogenesis and Mutagenesis |
| Issue number | SUPPL. 1 |
| Publication status | Published - 2003 |
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ASJC Scopus subject areas
- Oncology
- Genetics
- Genetics(clinical)
- Toxicology
- Health, Toxicology and Mutagenesis
Cite this
Repair of 8 oxoguanine in mammalian cells expressing the Drosophila S3 ribosomal/repair protein. / Cappelli, Enrico; D'Osualdo, Andrea; Bogliolo, Massimo; Xu, Yi; Kelley, Mark R.; Frosina, Guido.
In: Teratogenesis Carcinogenesis and Mutagenesis, No. SUPPL. 1, 2003, p. 113-121.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Repair of 8 oxoguanine in mammalian cells expressing the Drosophila S3 ribosomal/repair protein
AU - Cappelli, Enrico
AU - D'Osualdo, Andrea
AU - Bogliolo, Massimo
AU - Xu, Yi
AU - Kelley, Mark R.
AU - Frosina, Guido
PY - 2003
Y1 - 2003
N2 - 8-oxo-7,8-dihydroguanine (8-oxoG) is a potent mutagenic lesion that forms at elevated levels in cellular DNA and is repaired with low efficiency in human cells. Unlike its human counterpart, the Drosophila S3 ribosomal/repair protein is endowed with a vigorous 8 oxoG repair activity that is associated to β,δ-elimination AP lyase activity. We have recently observed that pure GST-tagged Drosophila S3 protein can significantly accelerate the in vitro repair of 8 oxoG performed by human and mouse cell extracts [Cappelli et al., unpublished data]. In this work, we have transfected Chinese hamster cells with mammalian expression vectors containing the Drosophila S3 cDNA. The cells synthesized both S3 mRNA and protein but no improved repair of 8 oxoguanine was observed. Factors important for the proper expression of Drosophila genes in mammalian cells are discussed.
AB - 8-oxo-7,8-dihydroguanine (8-oxoG) is a potent mutagenic lesion that forms at elevated levels in cellular DNA and is repaired with low efficiency in human cells. Unlike its human counterpart, the Drosophila S3 ribosomal/repair protein is endowed with a vigorous 8 oxoG repair activity that is associated to β,δ-elimination AP lyase activity. We have recently observed that pure GST-tagged Drosophila S3 protein can significantly accelerate the in vitro repair of 8 oxoG performed by human and mouse cell extracts [Cappelli et al., unpublished data]. In this work, we have transfected Chinese hamster cells with mammalian expression vectors containing the Drosophila S3 cDNA. The cells synthesized both S3 mRNA and protein but no improved repair of 8 oxoguanine was observed. Factors important for the proper expression of Drosophila genes in mammalian cells are discussed.
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UR - http://www.scopus.com/inward/citedby.url?scp=0037289231&partnerID=8YFLogxK
M3 - Article
C2 - 12616602
AN - SCOPUS:0037289231
SP - 113
EP - 121
JO - Teratogenesis Carcinogenesis and Mutagenesis
JF - Teratogenesis Carcinogenesis and Mutagenesis
SN - 0270-3211
IS - SUPPL. 1
ER -