Repeated administration of the food additive E171 to mice results in accumulation in intestine and liver and promotes an inflammatory status

Laura Talamini, Sara Gimondi, Martina B. Violatto, Fabio Fiordaliso, Federica Pedica, Ngoc Lan Tran, Giovanni Sitia, Federica Aureli, Andrea Raggi, Inge Nelissen, Francesco Cubadda, Paolo Bigini, Luisa Diomede

Research output: Contribution to journalArticle

Abstract

Titanium dioxide (TiO2) is widely used in pharmaceuticals preparations, cosmetics, and as a food additive (E171). It contains microparticles and a fraction of nanoparticles (NPs) which can be absorbed systemically by humans after ingestion. Increasing concern has been aroused about the impact of oral exposure to TiO2 NPs from dietary and non-dietary sources on human health. In spite of several toxicological studies conducted in recent years, a solid risk assessment of oral exposure to E171 has not been satisfactorily achieved. We investigated whether repeated oral administration of E171 to mice at a dose level (5mg/kg body weight for 3days/week for 3weeks) comparable to estimated human dietary exposure, results in TiO2 deposition in the digestive system and internal organs, and in molecular and cellular alterations associated with an inflammatory response. To reproduce the first phase of digestion, a new administration approach involving the dripping of the E171 suspension into the mouth of mice was applied. Significant accumulation of titanium was observed in the liver and intestine of E171-fed mice; in the latter a threefold increase in the number of TiO2 particles was also measured. Titanium accumulation in liver was associated with necroinflammatory foci containing tissue monocytes/macrophages. Three days after the last dose, increased superoxide production and inflammation were observed in the stomach and intestine. Overall, the present study indicates that the risk for human health associated with dietary exposure to E171 needs to be carefully considered.

Original languageEnglish
Pages (from-to)1087-1101
Number of pages15
JournalNanotoxicology
Volume13
Issue number8
DOIs
Publication statusPublished - Jan 1 2019

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Keywords

  • E171
  • gastrointestinal tract
  • inflammation
  • nanoparticles
  • Titanium dioxide

ASJC Scopus subject areas

  • Biomedical Engineering
  • Toxicology

Cite this

Talamini, L., Gimondi, S., Violatto, M. B., Fiordaliso, F., Pedica, F., Tran, N. L., Sitia, G., Aureli, F., Raggi, A., Nelissen, I., Cubadda, F., Bigini, P., & Diomede, L. (2019). Repeated administration of the food additive E171 to mice results in accumulation in intestine and liver and promotes an inflammatory status. Nanotoxicology, 13(8), 1087-1101. https://doi.org/10.1080/17435390.2019.1640910