Replication of association of CHRNA4 rare variants with sporadic amyotrophic lateral sclerosis: The Italian multicentre study

Mario Sabatelli, Serena Lattante, Amelia Conte, Giuseppe Marangi, Marco Luigetti, Alessandra Del Grande, Adriano Chiò, Massimo Corbo, Fabio Giannini, Jessica Mandrioli, Gabriele Mora, Andrea Calvo, Gabriella Restagno, Christian Lunetta, Silvana Penco, Stefania Battistini, Paolo Zeppilli, Alessandra Bizzarro, Ettore Capoluongo, Giovanni NeriPaolo Maria Rossini, Marcella Zollino

Research output: Contribution to journalArticle

Abstract

Neuronal nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels widely expressed throughout the mammalian brain, including bulbar and spinal motor neurons. They are involved in neuroprotection and in control of release of many neurotransmitters, including glutamate. Previous data raised the hypothesis that rare variants in the region coding the intracellular loop subunits of nAChRs might represent one of several genetic risk factors for SALS. The aim of present study was to replicate the study in an independent cohort of ALS patients. We analysed 718 sporadic ALS patients from five Italian ALS centres and 1300 ethnically matched controls. We focused primarily on CHRNA4, encoding α4 subunit, since most mutations were previously detected in this gene. We observed a significant association between CHRNA4 mutations and ALS (OR 2.91; 95% CI 1.4080-6.0453; p = 0.0056). Most mutations detected in patients were not present in the dbSNP134 and in 3500 ethnically matched control chromosomes and affected evolutionary conserved amino acid residues. In conclusion, the present data confirm that CHRNA4 variants are overrepresented in SALS strengthening the hypothesis can they act as predisposing genetic factors for SALS.

Original languageEnglish
Pages (from-to)580-584
Number of pages5
JournalAmyotrophic Lateral Sclerosis
Volume13
Issue number6
DOIs
Publication statusPublished - Oct 2012

Keywords

  • DNA
  • Genetics
  • Risk

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

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    Sabatelli, M., Lattante, S., Conte, A., Marangi, G., Luigetti, M., Del Grande, A., Chiò, A., Corbo, M., Giannini, F., Mandrioli, J., Mora, G., Calvo, A., Restagno, G., Lunetta, C., Penco, S., Battistini, S., Zeppilli, P., Bizzarro, A., Capoluongo, E., ... Zollino, M. (2012). Replication of association of CHRNA4 rare variants with sporadic amyotrophic lateral sclerosis: The Italian multicentre study. Amyotrophic Lateral Sclerosis, 13(6), 580-584. https://doi.org/10.3109/17482968.2012.704926