Abstract
Either aphidicolin- or thymidine-synchronized human HL-60 cells were used to study the replication pattern of a family of human repetitive DNA sequences, the EcoRI 340 bp family (αRI-DNA), and of the ladders of fragments generated in total human DNA after digestion with XbaI and HaeIII (alpha satellite sequences). DNAs replicated in early, middle-early, middle-late and late S periods were labelled with BUdR or with [3H]thymidine. The efficiency of the cell synchronization procedure was confirmed by the transition from a high-GC to a high-AT average base composition of the DNA synthesized going from early to late S periods. By hybridizing EcoRI 340 bp repetitive fragments to BUdR-DNAs it was found that this family of sequences is replicated throughout the entire S period. Comparing fluorograph densitometric scans of [3H]DNAs to the scans of ethidium bromide patterns of total HL-60 DNA digested with XbaI and HaeIII, it was observed that DNA synthesized in different S periods is characterized by approximately the same ladder of fragments, while the intensity of each band may vary through the S phase; in particular, the XbaI 2.4 kb fragment becomes undetectable in late S.
| Original language | English |
|---|---|
| Pages (from-to) | 53-59 |
| Number of pages | 7 |
| Journal | BBA - Gene Structure and Expression |
| Volume | 783 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Oct 5 1984 |
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Keywords
- Cell cycle
- DNA replication
- repetitive DNA
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Genetics
- Structural Biology
- Medicine(all)
Cite this
Replication pattern of human repeated DNA sequences. / Meneveri, Raffaella; Agresti, Alessandra; Breviario, Diego; Ginelli, Enrico.
In: BBA - Gene Structure and Expression, Vol. 783, No. 1, 05.10.1984, p. 53-59.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Replication pattern of human repeated DNA sequences
AU - Meneveri, Raffaella
AU - Agresti, Alessandra
AU - Breviario, Diego
AU - Ginelli, Enrico
PY - 1984/10/5
Y1 - 1984/10/5
N2 - Either aphidicolin- or thymidine-synchronized human HL-60 cells were used to study the replication pattern of a family of human repetitive DNA sequences, the EcoRI 340 bp family (αRI-DNA), and of the ladders of fragments generated in total human DNA after digestion with XbaI and HaeIII (alpha satellite sequences). DNAs replicated in early, middle-early, middle-late and late S periods were labelled with BUdR or with [3H]thymidine. The efficiency of the cell synchronization procedure was confirmed by the transition from a high-GC to a high-AT average base composition of the DNA synthesized going from early to late S periods. By hybridizing EcoRI 340 bp repetitive fragments to BUdR-DNAs it was found that this family of sequences is replicated throughout the entire S period. Comparing fluorograph densitometric scans of [3H]DNAs to the scans of ethidium bromide patterns of total HL-60 DNA digested with XbaI and HaeIII, it was observed that DNA synthesized in different S periods is characterized by approximately the same ladder of fragments, while the intensity of each band may vary through the S phase; in particular, the XbaI 2.4 kb fragment becomes undetectable in late S.
AB - Either aphidicolin- or thymidine-synchronized human HL-60 cells were used to study the replication pattern of a family of human repetitive DNA sequences, the EcoRI 340 bp family (αRI-DNA), and of the ladders of fragments generated in total human DNA after digestion with XbaI and HaeIII (alpha satellite sequences). DNAs replicated in early, middle-early, middle-late and late S periods were labelled with BUdR or with [3H]thymidine. The efficiency of the cell synchronization procedure was confirmed by the transition from a high-GC to a high-AT average base composition of the DNA synthesized going from early to late S periods. By hybridizing EcoRI 340 bp repetitive fragments to BUdR-DNAs it was found that this family of sequences is replicated throughout the entire S period. Comparing fluorograph densitometric scans of [3H]DNAs to the scans of ethidium bromide patterns of total HL-60 DNA digested with XbaI and HaeIII, it was observed that DNA synthesized in different S periods is characterized by approximately the same ladder of fragments, while the intensity of each band may vary through the S phase; in particular, the XbaI 2.4 kb fragment becomes undetectable in late S.
KW - Cell cycle
KW - DNA replication
KW - repetitive DNA
UR - http://www.scopus.com/inward/record.url?scp=0021755869&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021755869&partnerID=8YFLogxK
U2 - 10.1016/0167-4781(84)90077-0
DO - 10.1016/0167-4781(84)90077-0
M3 - Article
C2 - 6089891
AN - SCOPUS:0021755869
VL - 783
SP - 53
EP - 59
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 1
ER -