Reproducibility and accuracy of non-invasive measurement of infarct size in mice with high-resolution PET/CT

Adelaide Greco, Maria Piera Petretta, Michele Larobina, Sara Gargiulo, Mariarosaria Panico, Stephan G. Nekolla, Giovanni Esposito, Mario Petretta, Arturo Brunetti, Alberto Cuocolo

Research output: Contribution to journalArticle

Abstract

Background. This study assessed the reproducibility and accuracy of 2-deoxy-2[ 18F]fluoro-D-glucose ( 18F-FDG) for non-invasive quantification of myocardial infarct size in mice by a high-resolution positron emission tomography (PET)/computed tomography (CT) system. Methods and Results. Mice were studied by 18F-FDG PET/CT 1 week after induction of myocardial infarction by permanent coronary occlusion or sham procedure. In a subset of mice, PET/CT was repeated 2 days apart to assess the reproducibility of infarct size measurements. Histological analysis was used as reference method to validate imaging data. The average difference in infarct size measurements between the first and the second study was 20.42% ± 2.07% (95% confidence interval 22.6 to 1.75) with a repeatability coefficient of 4.05%. At Bland-Altman analysis, the lower and upper limits of agreement between the two repeated studies were 24.46% and 3.63%, respectively, and no correlation between difference and mean was found (P =.89). The concordance correlation coefficient was 0.99 (P >.001) and the intraclass coefficient of correlation was 0.99. A high correlation between PET/CT and histology was found for measurement of infarct size (P >.001). Using Bland-Altman analysis, the mean difference in infarct size measurement (PET/CT minus histology) was 1.9% (95% confidence interval 0.94% to 2.86%). Conclusions. In a micemodel of permanent coronary occlusion non-invasive measurement of infarct size with high-resolution 18F-FDG, PET/CT has excellent reproducibility and accuracy. These findings support the use of thismethodology in serial studies.

Original languageEnglish
Pages (from-to)492-499
Number of pages8
JournalJournal of Nuclear Cardiology
Volume19
Issue number3
DOIs
Publication statusPublished - Jun 2012

Keywords

  • F-FDG
  • Infarct size
  • Measurement
  • Mouse
  • PET/CT

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Radiology Nuclear Medicine and imaging

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    Greco, A., Petretta, M. P., Larobina, M., Gargiulo, S., Panico, M., Nekolla, S. G., Esposito, G., Petretta, M., Brunetti, A., & Cuocolo, A. (2012). Reproducibility and accuracy of non-invasive measurement of infarct size in mice with high-resolution PET/CT. Journal of Nuclear Cardiology, 19(3), 492-499. https://doi.org/10.1007/s12350-012-9538-5