Reproducibility patterns of plasma norepinephrine and muscle sympathetic nerve traffic in human obesity

G. Grassi, G. Seravalle, R. Dell'Oro, F. Arenare, R. Facchetti, G. Mancia

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background and aims: The present study was designed to assess the reproducibility of the two markers of adrenergic drive, venous plasma norepinephrine and efferent postganglionic muscle sympathetic nerve traffic (MSNA) in reflecting the sympathetic activation characterizing the obese state in human beings. Methods and results: In 15 male obese normotensive subjects (age: 40.1 ± 2.2, mean ± SEM) we measured, in two experimental sessions three weeks apart, blood pressure (BP, Finapres), heart rate (EKG), plasma norepinephrine (HPLC assay) and MSNA (microneurography, peroneal nerve). In each session three norepinephrine samples were obtained and norepinephrine reproducibility between sessions was assessed by considering a single norepinephrine sample or by averaging 2-3 samples. Reproducibility data were compared to the ones displayed by the MSNA technique. While MSNA values showed a highly significant correlation between sessions (r = 0.89, p <0.001), norepinephrine values based on a single blood sample evaluation did not correlate with each other (r = 0.44, p = NS). Norepinephrine correlation coefficient values increased and achieved statistical significance when average data from 3 blood samples were examined (r = 0.56, p <0.03). Conclusions: In human obesity MSNA displays a reproducibility pattern higher than plasma norepinephrine. The reproducibility of the norepinephrine approach can be improved by increasing the number of blood samples on which norepinephrine assay is performed. To obtain such a goal, and to make reproducibility closer to the MSNA one, three norepinephrine samples are needed.

Original languageEnglish
Pages (from-to)469-475
Number of pages7
JournalNutrition, Metabolism and Cardiovascular Diseases
Volume19
Issue number7
DOIs
Publication statusPublished - Sep 2009

Fingerprint

Norepinephrine
Obesity
Muscles
Peroneal Nerve
Adrenergic Agents
Electrocardiography
Heart Rate
High Pressure Liquid Chromatography
Blood Pressure

Keywords

  • Muscle sympathetic nerve traffic
  • Obesity
  • Plasma norepinephrine
  • Sympathetic activity

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Cardiology and Cardiovascular Medicine
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Reproducibility patterns of plasma norepinephrine and muscle sympathetic nerve traffic in human obesity. / Grassi, G.; Seravalle, G.; Dell'Oro, R.; Arenare, F.; Facchetti, R.; Mancia, G.

In: Nutrition, Metabolism and Cardiovascular Diseases, Vol. 19, No. 7, 09.2009, p. 469-475.

Research output: Contribution to journalArticle

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