Reprogramming of Oncogene Expression in Gingival Mesenchymal Stem Cells Following Long-Term Culture In Vitro

Agnese Gugliandolo, Thangavelu Soundara Rajan, Domenico Scionti, Francesca Diomede, Placido Bramanti, Emanuela Mazzon, Oriana Trubiani

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Mesenchymal stem cells (MSCs) are a promising resource for stem cell therapy for the treatment of different neurodegenerative disorders. In particular, dental MSCs, given their origin from neural crest and their proneness toward neuronal differentiation, may be more suitable for transplantation. However, if MSCs can undergo spontaneous transformation and give rise to tumor is still debated. Data about transcriptional regulation of oncogenes in MSCs following in vitro expansion are not available. In this work, we compared gene expression levels of oncogenes in gingival-derived MSCs at passage number 10 and 41. We found that the expression of 22 oncogenes was abolished in gingival MSCs at passage number 41 compared to those at passage number 10, and this may indicate a greater safety of high number passage MSCs.

Original languageEnglish
Pages (from-to)159-170
Number of pages12
JournalCellular Reprogramming
Volume19
Issue number3
DOIs
Publication statusPublished - Jun 2017

Fingerprint

Mesenchymal Stromal Cells
Oncogenes
Neural Crest
Cell- and Tissue-Based Therapy
In Vitro Techniques
Neurodegenerative Diseases
Tooth
Stem Cells
Transplantation
Safety
Gene Expression
Neoplasms

Keywords

  • Journal Article

Cite this

Reprogramming of Oncogene Expression in Gingival Mesenchymal Stem Cells Following Long-Term Culture In Vitro. / Gugliandolo, Agnese; Rajan, Thangavelu Soundara; Scionti, Domenico; Diomede, Francesca; Bramanti, Placido; Mazzon, Emanuela; Trubiani, Oriana.

In: Cellular Reprogramming, Vol. 19, No. 3, 06.2017, p. 159-170.

Research output: Contribution to journalArticle

Gugliandolo, Agnese ; Rajan, Thangavelu Soundara ; Scionti, Domenico ; Diomede, Francesca ; Bramanti, Placido ; Mazzon, Emanuela ; Trubiani, Oriana. / Reprogramming of Oncogene Expression in Gingival Mesenchymal Stem Cells Following Long-Term Culture In Vitro. In: Cellular Reprogramming. 2017 ; Vol. 19, No. 3. pp. 159-170.
@article{e5681806b60144319dd514d7f1ce99d3,
title = "Reprogramming of Oncogene Expression in Gingival Mesenchymal Stem Cells Following Long-Term Culture In Vitro",
abstract = "Mesenchymal stem cells (MSCs) are a promising resource for stem cell therapy for the treatment of different neurodegenerative disorders. In particular, dental MSCs, given their origin from neural crest and their proneness toward neuronal differentiation, may be more suitable for transplantation. However, if MSCs can undergo spontaneous transformation and give rise to tumor is still debated. Data about transcriptional regulation of oncogenes in MSCs following in vitro expansion are not available. In this work, we compared gene expression levels of oncogenes in gingival-derived MSCs at passage number 10 and 41. We found that the expression of 22 oncogenes was abolished in gingival MSCs at passage number 41 compared to those at passage number 10, and this may indicate a greater safety of high number passage MSCs.",
keywords = "Journal Article",
author = "Agnese Gugliandolo and Rajan, {Thangavelu Soundara} and Domenico Scionti and Francesca Diomede and Placido Bramanti and Emanuela Mazzon and Oriana Trubiani",
year = "2017",
month = "6",
doi = "10.1089/cell.2016.0056",
language = "English",
volume = "19",
pages = "159--170",
journal = "Cellular Reprogramming",
issn = "2152-4971",
publisher = "Mary Ann Liebert Inc.",
number = "3",

}

TY - JOUR

T1 - Reprogramming of Oncogene Expression in Gingival Mesenchymal Stem Cells Following Long-Term Culture In Vitro

AU - Gugliandolo, Agnese

AU - Rajan, Thangavelu Soundara

AU - Scionti, Domenico

AU - Diomede, Francesca

AU - Bramanti, Placido

AU - Mazzon, Emanuela

AU - Trubiani, Oriana

PY - 2017/6

Y1 - 2017/6

N2 - Mesenchymal stem cells (MSCs) are a promising resource for stem cell therapy for the treatment of different neurodegenerative disorders. In particular, dental MSCs, given their origin from neural crest and their proneness toward neuronal differentiation, may be more suitable for transplantation. However, if MSCs can undergo spontaneous transformation and give rise to tumor is still debated. Data about transcriptional regulation of oncogenes in MSCs following in vitro expansion are not available. In this work, we compared gene expression levels of oncogenes in gingival-derived MSCs at passage number 10 and 41. We found that the expression of 22 oncogenes was abolished in gingival MSCs at passage number 41 compared to those at passage number 10, and this may indicate a greater safety of high number passage MSCs.

AB - Mesenchymal stem cells (MSCs) are a promising resource for stem cell therapy for the treatment of different neurodegenerative disorders. In particular, dental MSCs, given their origin from neural crest and their proneness toward neuronal differentiation, may be more suitable for transplantation. However, if MSCs can undergo spontaneous transformation and give rise to tumor is still debated. Data about transcriptional regulation of oncogenes in MSCs following in vitro expansion are not available. In this work, we compared gene expression levels of oncogenes in gingival-derived MSCs at passage number 10 and 41. We found that the expression of 22 oncogenes was abolished in gingival MSCs at passage number 41 compared to those at passage number 10, and this may indicate a greater safety of high number passage MSCs.

KW - Journal Article

U2 - 10.1089/cell.2016.0056

DO - 10.1089/cell.2016.0056

M3 - Article

C2 - 28440665

VL - 19

SP - 159

EP - 170

JO - Cellular Reprogramming

JF - Cellular Reprogramming

SN - 2152-4971

IS - 3

ER -